Fig. 5

Subcellular localization of wild type and mutated OsCSP2 in the rice roots and BiFC analysis. a Subcellular localization of WtOsCSP2-sGFP fusion protein in a rice root cell (a). DAPI staining of (a) is shown in (b). A merged image of (a) and (b) is shown in (c). Bright field of (a) is in (d). A merged image of (a) and (d) is shown in (e). Mutated OsCSP2 (C140S)-sGFP showed abnormal localization in a rice root cell (f). DAPI staining of (f) is shown in (g). A merged image of (f) and (g) is shown in (h). Bright field of (f) is in (i). A merged image of (f) and (i) is shown in (j). Arrowheads indicate the localization of observed GFP signal. Scale bar represents 50 μm. b BiFC analysis of WtOsCSP2 is shown in (a). Bright field of (a) is in (b). A merged image of (a) and (b) is shown in (c). BiFC analysis of mutated OsCSP2 (C140S) is shown in (d). Bright field of (d) is in (e). A merged image of (d) and (e) is shown in (f). Arrowheads indicate the localization of observed BiFC (mKG) signal. Scale bar represents 50 μm