Fig. 4

Representative 2-DE gel image of proteins in response to water deficit from C. humilileaves. a control; b 12 days after water deficit; c 24 days after water deficit. Proteins were separated on 13 cm IPG strips (pI 4-7 linear gradient) using IEF in the first dimension, followed by 12.5% SDS-PAGE gels in the second dimension. The 2-DE gel was stained with Coomassie Brilliant Blue. Molecular weight (MW) in kDa and pI of proteins are indicated on the left and top of the gel, respectively. A total of 46 differentially expressed proteins identified by MALDI-TOF-MS/MS were marked with numbers on the gel, and detailed information can be found in Additional file 2: Table S2 and Table 1