Fig. 1

Coomassie-stained 1D SDS-PAGE gels used in first in-gel digestion. Fractions of acetone precipitated serum protein from a healthy sheep were loaded alongside bovine serum albumin (BSA) in Gel A. Gel A suffered a handling artefact to the top right corner of the gel. The leftmost well of both gels were loaded with 4 μL of a protein molecular weight standard (Precision Plus Protein™ Dual Xtra, Bio-Rad Laboratories). One well in Gel A was loaded with 500 fm of BSA standard; other three wells were loaded with 22 μg, 10 μg and 2 μg each of sheep serum protein sample. After the molecular weight standard, the other three wells in Gel B were loaded with 500 fm, 2500 fm and 250 fm each of BSA standard. Arrows show BSA standard