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Table 1 The number of proteins identified by ProteinPilotâ„¢ Software from in-gel and in-solution digestion of healthy sheep serum samples by searching a composite Ovis aries, Bos taurus and Capra hircus protein sequence database

From: Characterisation of the circulating acellular proteome of healthy sheep using LC-MS/MS-based proteomics analysis of serum

Experiment →

First digestion

Second digestion

Third digestion

Digestion type

In-gel

In-sol

In-gel

In-sol

In-gel

In-sol

Serum protein source

Ac

Ac

Ac

Crude

Ac + Crude

Crude

Total quantity of protein analysed

34 μg

10 μg

350 μg

20 μg

900 μg

100 μg

Number of protein IDs

120

25

241

100

182

32

  1. Key: In-sol In-solution; Ac Acetone precipitated; IDs Identifications