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Table 1 Photophysical properties of fluorogenic tetrazine fluorophores

From: Tetrazine ligation for chemical proteomics

probe Ex/Em (nm) ε (M−1cm−1) Φ Fluorescence
Enhancement
HELIOS347 347/455 18500 0.29 2500
HELIOS370 370/463 19000 0.49 2900
HELIOS380 388/482 20000 0.38 11000
HELIOS400 400/502 16000 0.41 4000
2b NA NA 0.80 900
4b NA NA 0.73 1600
5b NA NA ND ND
Fl-5-Tz 495/521 57000 0.0037 72
Fl-6-Tz 495/517 55000 0.0033 109
OG-5-Tz 495/524 87000 0.0048 60
OG-6-Tz 495/522 70000 0.0041 103
Rh-5-Tz 556/580 46000 0.037 22
Rh-6-Tz 554/577 54000 0.031 12
SiRh-5-Tz 650/665 58000 0.020 1.8
SiRh-6-Tz 649/664 62000 0.017 3.7
MeSiRh-5-Tz 651/666 53000 0.026 2.0