Table 1 Photophysical properties of fluorogenic tetrazine fluorophores
probe | Ex/Em (nm) | ε (M−1cm−1) | Φ | Fluorescence Enhancement |
|---|---|---|---|---|
HELIOS347 | 347/455 | 18500 | 0.29 | 2500 |
HELIOS370 | 370/463 | 19000 | 0.49 | 2900 |
HELIOS380 | 388/482 | 20000 | 0.38 | 11000 |
HELIOS400 | 400/502 | 16000 | 0.41 | 4000 |
2b | NA | NA | 0.80 | 900 |
4b | NA | NA | 0.73 | 1600 |
5b | NA | NA | ND | ND |
Fl-5-Tz | 495/521 | 57000 | 0.0037 | 72 |
Fl-6-Tz | 495/517 | 55000 | 0.0033 | 109 |
OG-5-Tz | 495/524 | 87000 | 0.0048 | 60 |
OG-6-Tz | 495/522 | 70000 | 0.0041 | 103 |
Rh-5-Tz | 556/580 | 46000 | 0.037 | 22 |
Rh-6-Tz | 554/577 | 54000 | 0.031 | 12 |
SiRh-5-Tz | 650/665 | 58000 | 0.020 | 1.8 |
SiRh-6-Tz | 649/664 | 62000 | 0.017 | 3.7 |
MeSiRh-5-Tz | 651/666 | 53000 | 0.026 | 2.0 |