UAA functionalities | Reaction partner | Reaction rate (M−1 s−1) | Condition | Labeled Protein | system | Ref |
---|---|---|---|---|---|---|
Tetrazine | s-TCO | 880 ± 10 (in vitro) 330 ± 20 (in E. coli) | E. coli. | GFP | tRNACUA/MjTyrRS (Y32E, L65A, A107E, F108P, Q109S, D158G, L162G) | [43] |
trans-cyclooctene | dipyridyl-Tz | 17248 ± 3132 (in vitro) | E. coli., HEK293 | sfGFP | tRNACUA/MbPylRS (Y271A, L274M, C313A) | [46] |
Tz-Cy5, Tz-TAMRA | NA 35000 ± 3000 | HeLa | GFP | tRNAPyl/PylRS(Y306A, Y384F) | [45] | |
norbornene | Tz-TAMRA | 9 | E. coli., HEK293 | sfGFP | tRNACUA/PylRS | [44] |
dipyridyl-Tz-DANSYL | NP | E. coli. | YFP | tRNAPyl/PylRS(Y394F,Y306G, I405R) | [77] | |
Tz-TAMRA | 8 | E. coil., HeLa | GFP | tRNAPyl/PylRS(Y306A, Y384F) | [45] | |
BCN | dipyridyl-Tz | 1245 ± 45 (in vitro) | E. coli., HEK293 | sfGFP | tRNACUA/MbPylRS (Y271M, L274G, C313A) | [46] |