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Table 2 Unnatural amino acids for iEDDA reaction

From: Tetrazine ligation for chemical proteomics

UAA functionalities

Reaction partner

Reaction rate (M−1 s−1)

Condition

Labeled Protein

system

Ref

Tetrazine

s-TCO

880 ± 10 (in vitro) 330 ± 20 (in E. coli)

E. coli.

GFP

tRNACUA/MjTyrRS (Y32E, L65A, A107E, F108P, Q109S, D158G, L162G)

[43]

trans-cyclooctene

dipyridyl-Tz

17248 ± 3132 (in vitro)

E. coli., HEK293

sfGFP

tRNACUA/MbPylRS (Y271A, L274M, C313A)

[46]

Tz-Cy5, Tz-TAMRA

NA 35000 ± 3000

HeLa

GFP

tRNAPyl/PylRS(Y306A, Y384F)

[45]

norbornene

Tz-TAMRA

9

E. coli., HEK293

sfGFP

tRNACUA/PylRS

[44]

dipyridyl-Tz-DANSYL

NP

E. coli.

YFP

tRNAPyl/PylRS(Y394F,Y306G, I405R)

[77]

Tz-TAMRA

8

E. coil., HeLa

GFP

tRNAPyl/PylRS(Y306A, Y384F)

[45]

BCN

dipyridyl-Tz

1245 ± 45 (in vitro)

E. coli., HEK293

sfGFP

tRNACUA/MbPylRS (Y271M, L274G, C313A)

[46]