Fig. 36

(top) Schematic of photo-affinity-based target identification (ID) with different photoactivatable linkers. Each target ID probe containing a photoactivatable moiety (BP, DA and AA) which can bind to a specific set of proteins in a structure-dependent manner. Figure reproduced from ref. [102] with permission from ACS publication. (Bottom) Schematic illustration of the molecular shape-dependence of protein labeling. The flexibility of linear molecules increases the binding to various proteins. Branched molecules bind to fewer proteins than linear molecules due to their restricted conformational flexibility. Figure reproduced from ref. [103] with permission from RSC publication