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Table 2 DNA sequencing of PCR products obtained from individual plaques after nine rounds of selection with B-DAP immobilized on a neutravidin-coated PS microtiter plate. The DNA sequences of each plaque are available upon request

From: Daptomycin, a last-resort antibiotic, binds ribosomal protein S19 in humans

Library

Plaques

Gene Identified from DNA Sequence

Notes

Frame

Cola

A3

leukocyte receptor cluster (LRC) member 8

minor fraction of CDS

1

Cola

D1

tenascin C (TNC)

500 bp of 6400 bp CDS

1

CoT

A4, E4, G4, H4

ribosomal protein S19

350 bp of 420 bp CDS

1

CoT

B4

dynamin 2 (DNM2)

only late 180 bp of 2500 bp CDS

2

CoT

B5

sphingomyelin phosphodiesterase 4

outside CDS

-

LiT

C1, D1, C2, H3

ribosomal protein S19

350 bp of 420 bp CDS

1

LiT

E1

ribosomal protein S19

360 bp of 420 bp CDS

1

LiT

B2

required for meiotic nuclear division 5 homolog B

gene in backwards

-

LiT

A3

UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5

gene in backwards

-

LiT

B3

RAD21 homolog (S. pombe)

500 bp of 1800 bp CDS

1

LuT

A4, C5

ribosomal protein S10

last 360 bp of 500 bp CDS

1

LuT

C4

ribosomal protein S19

300 bp of 420 bp CDS

1

BrT

B7

v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro

very late 480 bp of 3680 bp CDS

1

BrT

D7

ribosomal protein S19

300 bp of 420 bp CDS

1

BrT

A9

ribosomal protein S19

330 bp of 420 bp CDS

1

BrT

D9

ribosomal protein S10

430 bp of 500 bp CDS

1

BrT

E9

BAC clone RP11-22 K23

outside CDS

-

BrT

H9

LYR motif containing 5

230 bp of 270 bp CDS

1

  1. a Plaques derived from this library were picked after 12 rounds of selection