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Table 2 Identification of salt-resposive proteins in alfalfa using MALDI-TOF-MS/MS

From: Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots

Spot No.a Homologous protein (plant species)b gi Numberc Theo.
Mr./pId
Exp.
Mr./pIe
Scoresf M.Pg Relative Vol% ± SEh
CK 100 mM 200 mM
Photosynthesis
2 RuBisCO large subunit [Medicago sativa] 1,223,773 12.0/6.1 41.7/6.9 263 3
3 RuBisCO small subunit [Medicago sativa] 2,342,980 52.3/6.1 27.5/6.7 132 3
4 RuBisCO large subunit [Medicago sativa] 1,223,773 12.0/6.1 31.5/6.1 99 4
8 RuBisCO activase [Oryza sativa Japonica Group] 1,778,414 24.6/8.6 21.7/6.1 126 5
12 Cytochrome b6-f complex iron-sulfur [Pisum sativum] 136,707 23.1/5.9 23.5/5.7 138 5
16 Chlorophyll a/b binding protein [Arabidopsis thaliana] 16,374 25.0/5.1 36.6/4.5 112 4
26 Chloroplast oxygen-evolving enhancer protein 1 [Leymus chinensis] 147,945,622 34.5/6.0 35.5/6.1 95 4
6 CP12 [Chlamydomonas reinhardtii] 3,123,345 12.5/5.7 15.3/6.6 92 3
Stress and defense
25 Glutathione peroxidase 1 [Lotus japonicus] 37,930,463 26.0/10.0 31.8/4.6 97 7
23 Chloroplast thylakoid-bound ascorbate peroxidase [Vigna unguiculata] 45,268,437 45.1/8.2 31.2/5.8 115 4
13 Harvest-induced protein [Medicago sativa] 283,831,548 16.6/5.1 21.6/5.1 133 5
19 Quinone reductase family protein [Arabidopsis thaliana] 30,687,535 21.7/6.1 23.7/5.5 186 6
20 Pathogenesis-related protein 2 [Medicago sativa] 22,266,001 16.5/5.8 20.1/5.3 344 9
R4 Glutathione peroxidase [Medicago truncatula] 355,524,544 21.3/7.6 11.7/4.6 76 5
R21 Ascorbate peroxidase [Medicago sativa] 16,304,410 20.1/5.3 34.7/4.3 99 6
R23 Ascorbate peroxides [Medicago sativa] 16,304,410 20.1/5.3 40.1/4.2 81 5
R16 Cytosolic ascorbate peroxidase [Cucumis sativus] 1,669,585 27.5/5.4 37.9/6.1 102 3
R9 Pathogenesis-related protein 5 [Arabidopsis thaliana] 15,222,089 26.2/4.8 34.7/6.9 113 5
R27 Pathogenesis-related protein 2 [Medicago sativa] 22,266,001 16.5/5.8 23.7/5.6 344 9
R26 Alcohol dehydrogenase-1F [Phaseolus acutifolius] 113,361 41.8/6.1 21.3/5.1 101 4
R10 Alcohol dehydrogenase [Dianthus caryophyllus] 33,149,683 41.9/6.6 38.1/6.4 79 3
R18 Ferritin [Glycine max] 968,987 28.0/5.9 34.2/5.1 82 4
Carbohydrate and energy metabolism
18 Glyceraldehyde-3-phosphate dehydrogenases [Arabidopsis thaliana] 15,229,231 39.0/6.7 39.5/5.4 117 3
11 ATP synthase bate subunit [Kerria japonica] 7,578,491 48.0/5.5 23.2/5.6 101 5
R7 Malate dehydrogenase, mitochondrial
[Medicago sativa]
32,328,905 48.6/6.5 18.7/6.9 97 3
R34 Glyceraldehyde 3-phosphate dehydrogenases [Arabidopsis thaliana] 15,229,231 39.0/6.7 22.3/5.6 87 4
R14 ATP synthase bate china 2 mitochondrial [Arabidopsis thaliana] 18,415,911 73.0/5.8 42.3/6.7 75 4
R12 Nucleoside diphosphate kinase 1 [Pisum sativum] 134,667 16.4/5.9 95.0/6.5 69 3
R24 Cytosolic phosphoglycerate kinase [Pisum sativum] 923,077 42.3/5.7 91.2/5.1 522 9
Second metabolism
1 Allene oxide cyclase 2 [Arabidopsis thaliana] 18,404,656 38.9/ 5.0 42.7/6.9 91 5
17 Myo-inositol-3-phosphate synthase [Glycine max] 13,936,691 56.6/5.3 51.3/5.8 87 4
R5 Isoliquiritigenin 2′-O-methyltransferase [Medicago sativa] 44,887,779 41.5/5.1 25.0/4.3 88 5
R2 Glutamine synthetase [Arabidopsis thaliana] 28,393,681 44.0/5.15 18.5/4.5 121 5
R11 3-Isopropylmalate dehydrogenase [Arabidopsis thaliana] 121,343 46.6/6.0 38.0/6.0 104 4
R13 Chalcone reductase [Medicago sativa] 563,540 35.0/6.5 42.1/6.6 111 6
R19 Isoflavone reductase [Medicago sativa] 19,620 35.5/5.3 38.5/4.7 129 5
R33 Chalcone isomerase [Medicago sativa] 166,400 21.4/5.5 22.3/6.6 104 5
Protein metabolism
10 Small ribosomal protein 4 [Squamidium brasiliense] 37,992,679 21.9/10.0 23.3/5.8 79 3
R25 Probable protein disulfide-isomerase A6 [Arabidopsis thaliana] 7,294,421 40.8/5.4 22.3/5.7 544 9
R32 Ribosomal protein L32 [Medicago sativa] 71,534,997 15.7/10.9 30.2/6.8 69 5
R29 Mitochondrial processing peptidase [Solanum tuberosum] 587,562 54.6/5.9 44.2/6.0 72 4
R1 Heat shock protein 70 [Cucumis sativus] 1,143,427 75.3/5.1 12.0/4.4 75 4
R30 Proteasome subunit alpha type-2-B [Arabidopsis thaliana] 15,219,317 25.7/5.5 46.2/6.4 78 4
R28 Eukaryotic translation initiation factor 5A-2(eIF-5A-2) [Gossypium barbadense] 45,644,510 17.2/5.2 21.3/5.6 75 5
Transcriptional regulation
7 mRNA binding protein precursor [Solanum lycopersicum] 26,453,355 44.0/7.1 21.2/6.3 84 3
15 Nucleic acid binding protein1 [Zea mays] 162,463,757 33.1/4.6 33.1/4.4 102 5
5 Maturase [Kopsia fruticosa] 59,932,902 59.8/9.2 20.0/4.4 88 4
24 Putative RNA binding protein [Mesembryanthemum crystallinum] 388,621 32.0/4.7 43.7/5.7 87 4
R20 Putative polyprotein [Oryza sativa Japonica Group] 50,300,539 35.6/4.5 35.6/4.5 93 5
R22 RNA-binding protein [Arabidopsis thaliana] 21,593,201 42.7/7.7 34.2/4.4 85 5
R35 Nucleic acid binding protein1 [Zea mays] 162,463,757 33.1/4.6 48.8/6.0 89 4
Cell wall and cytoskeleton metabolism
21 Cell division cycle protein 48 [Arabidopsis thaliana] 1,841,493 90.6/5.0 33.0/4.5 81 5
22 Cell division cycle protein 48 [Arabidopsis thaliana] 1,841,493 90.6/5.0 32.8/4.5 73 5
R6 Actin-depolymerizing factor [Malus x domestica] 33,772,153 11.1/8.7 20.8/6.7 75 4
R8 Profilin-2 [Lilium longiflorum] 14,423,862 14.3/4.6 31.2/6.8 226 8
R31 Actin-depolymerizing factor [Malus domestica] 33,772,153 11.1/8.7 44.9/6.3 107 6
Signaling
R17 Annexin [Lavatera thuringiaca] 2,459,926 36.2/6.1 44.7/5.3 121 6
R15 Vacuole-associated annexin VCaB42 [Nicotiana tabacum] 4,580,920 36.0/5.3 37.7/5.7 135 5
Ion transport
R3 Plasma membrane H+- ATPase [Hordeum vulgare subsp. vulgare] 15,149,829 70.9/8.6 16.4/5.2 91 4
Unknown
9 Unknown protein [Medicago truncatula] 217,071,692 55.5/5.5 21.8/6.1 87 4
14 Unknown protein [Arabidopsis thaliana] 22,329,503 54.9/5.5 34.2/4.8 145 5
  1. aThe number of identification spot
  2. bThe homologous protein and plant species
  3. cThe number NCBInr databases
  4. dTheoretical mass (Mr, kDa) and pI of identified proteins. Theoretical values were retrieved from the protein database
  5. eExperimental mass (Mr, kDa) and pI of identified proteins. Experimental values were calculated with Image master software (GE Healthcare) and standard molecular mass markers
  6. fThe mascot scores
  7. gNumber of matched petides
  8. hThe Relative Vol% of spot