Table 2 Identification of salt-resposive proteins in alfalfa using MALDI-TOF-MS/MS
From: Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots
Spot No.a | Homologous protein (plant species)b | gi Numberc | Theo. Mr./pId | Exp. Mr./pIe | Scoresf | M.Pg | Relative Vol% ± SEh CK 100 mM 200 mM |
|---|---|---|---|---|---|---|---|
Photosynthesis | |||||||
2 | RuBisCO large subunit [Medicago sativa] | 1,223,773 | 12.0/6.1 | 41.7/6.9 | 263 | 3 |
|
3 | RuBisCO small subunit [Medicago sativa] | 2,342,980 | 52.3/6.1 | 27.5/6.7 | 132 | 3 |
|
4 | RuBisCO large subunit [Medicago sativa] | 1,223,773 | 12.0/6.1 | 31.5/6.1 | 99 | 4 |
|
8 | RuBisCO activase [Oryza sativa Japonica Group] | 1,778,414 | 24.6/8.6 | 21.7/6.1 | 126 | 5 |
|
12 | Cytochrome b6-f complex iron-sulfur [Pisum sativum] | 136,707 | 23.1/5.9 | 23.5/5.7 | 138 | 5 |
|
16 | Chlorophyll a/b binding protein [Arabidopsis thaliana] | 16,374 | 25.0/5.1 | 36.6/4.5 | 112 | 4 |
|
26 | Chloroplast oxygen-evolving enhancer protein 1 [Leymus chinensis] | 147,945,622 | 34.5/6.0 | 35.5/6.1 | 95 | 4 |
|
6 | CP12 [Chlamydomonas reinhardtii] | 3,123,345 | 12.5/5.7 | 15.3/6.6 | 92 | 3 |
|
Stress and defense | |||||||
25 | Glutathione peroxidase 1 [Lotus japonicus] | 37,930,463 | 26.0/10.0 | 31.8/4.6 | 97 | 7 |
|
23 | Chloroplast thylakoid-bound ascorbate peroxidase [Vigna unguiculata] | 45,268,437 | 45.1/8.2 | 31.2/5.8 | 115 | 4 |
|
13 | Harvest-induced protein [Medicago sativa] | 283,831,548 | 16.6/5.1 | 21.6/5.1 | 133 | 5 |
|
19 | Quinone reductase family protein [Arabidopsis thaliana] | 30,687,535 | 21.7/6.1 | 23.7/5.5 | 186 | 6 |
|
20 | Pathogenesis-related protein 2 [Medicago sativa] | 22,266,001 | 16.5/5.8 | 20.1/5.3 | 344 | 9 |
|
R4 | Glutathione peroxidase [Medicago truncatula] | 355,524,544 | 21.3/7.6 | 11.7/4.6 | 76 | 5 |
|
R21 | Ascorbate peroxidase [Medicago sativa] | 16,304,410 | 20.1/5.3 | 34.7/4.3 | 99 | 6 |
|
R23 | Ascorbate peroxides [Medicago sativa] | 16,304,410 | 20.1/5.3 | 40.1/4.2 | 81 | 5 |
|
R16 | Cytosolic ascorbate peroxidase [Cucumis sativus] | 1,669,585 | 27.5/5.4 | 37.9/6.1 | 102 | 3 |
|
R9 | Pathogenesis-related protein 5 [Arabidopsis thaliana] | 15,222,089 | 26.2/4.8 | 34.7/6.9 | 113 | 5 |
|
R27 | Pathogenesis-related protein 2 [Medicago sativa] | 22,266,001 | 16.5/5.8 | 23.7/5.6 | 344 | 9 |
|
R26 | Alcohol dehydrogenase-1F [Phaseolus acutifolius] | 113,361 | 41.8/6.1 | 21.3/5.1 | 101 | 4 |
|
R10 | Alcohol dehydrogenase [Dianthus caryophyllus] | 33,149,683 | 41.9/6.6 | 38.1/6.4 | 79 | 3 |
|
R18 | Ferritin [Glycine max] | 968,987 | 28.0/5.9 | 34.2/5.1 | 82 | 4 |
|
Carbohydrate and energy metabolism | |||||||
18 | Glyceraldehyde-3-phosphate dehydrogenases [Arabidopsis thaliana] | 15,229,231 | 39.0/6.7 | 39.5/5.4 | 117 | 3 |
|
11 | ATP synthase bate subunit [Kerria japonica] | 7,578,491 | 48.0/5.5 | 23.2/5.6 | 101 | 5 |
|
R7 | Malate dehydrogenase, mitochondrial [Medicago sativa] | 32,328,905 | 48.6/6.5 | 18.7/6.9 | 97 | 3 |
|
R34 | Glyceraldehyde 3-phosphate dehydrogenases [Arabidopsis thaliana] | 15,229,231 | 39.0/6.7 | 22.3/5.6 | 87 | 4 |
|
R14 | ATP synthase bate china 2 mitochondrial [Arabidopsis thaliana] | 18,415,911 | 73.0/5.8 | 42.3/6.7 | 75 | 4 |
|
R12 | Nucleoside diphosphate kinase 1 [Pisum sativum] | 134,667 | 16.4/5.9 | 95.0/6.5 | 69 | 3 |
|
R24 | Cytosolic phosphoglycerate kinase [Pisum sativum] | 923,077 | 42.3/5.7 | 91.2/5.1 | 522 | 9 |
|
Second metabolism | |||||||
1 | Allene oxide cyclase 2 [Arabidopsis thaliana] | 18,404,656 | 38.9/ 5.0 | 42.7/6.9 | 91 | 5 |
|
17 | Myo-inositol-3-phosphate synthase [Glycine max] | 13,936,691 | 56.6/5.3 | 51.3/5.8 | 87 | 4 |
|
R5 | Isoliquiritigenin 2′-O-methyltransferase [Medicago sativa] | 44,887,779 | 41.5/5.1 | 25.0/4.3 | 88 | 5 |
|
R2 | Glutamine synthetase [Arabidopsis thaliana] | 28,393,681 | 44.0/5.15 | 18.5/4.5 | 121 | 5 |
|
R11 | 3-Isopropylmalate dehydrogenase [Arabidopsis thaliana] | 121,343 | 46.6/6.0 | 38.0/6.0 | 104 | 4 |
|
R13 | Chalcone reductase [Medicago sativa] | 563,540 | 35.0/6.5 | 42.1/6.6 | 111 | 6 |
|
R19 | Isoflavone reductase [Medicago sativa] | 19,620 | 35.5/5.3 | 38.5/4.7 | 129 | 5 |
|
R33 | Chalcone isomerase [Medicago sativa] | 166,400 | 21.4/5.5 | 22.3/6.6 | 104 | 5 |
|
Protein metabolism | |||||||
10 | Small ribosomal protein 4 [Squamidium brasiliense] | 37,992,679 | 21.9/10.0 | 23.3/5.8 | 79 | 3 |
|
R25 | Probable protein disulfide-isomerase A6 [Arabidopsis thaliana] | 7,294,421 | 40.8/5.4 | 22.3/5.7 | 544 | 9 |
|
R32 | Ribosomal protein L32 [Medicago sativa] | 71,534,997 | 15.7/10.9 | 30.2/6.8 | 69 | 5 |
|
R29 | Mitochondrial processing peptidase [Solanum tuberosum] | 587,562 | 54.6/5.9 | 44.2/6.0 | 72 | 4 |
|
R1 | Heat shock protein 70 [Cucumis sativus] | 1,143,427 | 75.3/5.1 | 12.0/4.4 | 75 | 4 |
|
R30 | Proteasome subunit alpha type-2-B [Arabidopsis thaliana] | 15,219,317 | 25.7/5.5 | 46.2/6.4 | 78 | 4 |
|
R28 | Eukaryotic translation initiation factor 5A-2(eIF-5A-2) [Gossypium barbadense] | 45,644,510 | 17.2/5.2 | 21.3/5.6 | 75 | 5 |
|
Transcriptional regulation | |||||||
7 | mRNA binding protein precursor [Solanum lycopersicum] | 26,453,355 | 44.0/7.1 | 21.2/6.3 | 84 | 3 |
|
15 | Nucleic acid binding protein1 [Zea mays] | 162,463,757 | 33.1/4.6 | 33.1/4.4 | 102 | 5 |
|
5 | Maturase [Kopsia fruticosa] | 59,932,902 | 59.8/9.2 | 20.0/4.4 | 88 | 4 |
|
24 | Putative RNA binding protein [Mesembryanthemum crystallinum] | 388,621 | 32.0/4.7 | 43.7/5.7 | 87 | 4 |
|
R20 | Putative polyprotein [Oryza sativa Japonica Group] | 50,300,539 | 35.6/4.5 | 35.6/4.5 | 93 | 5 |
|
R22 | RNA-binding protein [Arabidopsis thaliana] | 21,593,201 | 42.7/7.7 | 34.2/4.4 | 85 | 5 |
|
R35 | Nucleic acid binding protein1 [Zea mays] | 162,463,757 | 33.1/4.6 | 48.8/6.0 | 89 | 4 |
|
Cell wall and cytoskeleton metabolism | |||||||
21 | Cell division cycle protein 48 [Arabidopsis thaliana] | 1,841,493 | 90.6/5.0 | 33.0/4.5 | 81 | 5 |
|
22 | Cell division cycle protein 48 [Arabidopsis thaliana] | 1,841,493 | 90.6/5.0 | 32.8/4.5 | 73 | 5 |
|
R6 | Actin-depolymerizing factor [Malus x domestica] | 33,772,153 | 11.1/8.7 | 20.8/6.7 | 75 | 4 |
|
R8 | Profilin-2 [Lilium longiflorum] | 14,423,862 | 14.3/4.6 | 31.2/6.8 | 226 | 8 |
|
R31 | Actin-depolymerizing factor [Malus domestica] | 33,772,153 | 11.1/8.7 | 44.9/6.3 | 107 | 6 |
|
Signaling | |||||||
R17 | Annexin [Lavatera thuringiaca] | 2,459,926 | 36.2/6.1 | 44.7/5.3 | 121 | 6 |
|
R15 | Vacuole-associated annexin VCaB42 [Nicotiana tabacum] | 4,580,920 | 36.0/5.3 | 37.7/5.7 | 135 | 5 |
|
Ion transport | |||||||
R3 | Plasma membrane H+- ATPase [Hordeum vulgare subsp. vulgare] | 15,149,829 | 70.9/8.6 | 16.4/5.2 | 91 | 4 |
|
Unknown | |||||||
9 | Unknown protein [Medicago truncatula] | 217,071,692 | 55.5/5.5 | 21.8/6.1 | 87 | 4 |
|
14 | Unknown protein [Arabidopsis thaliana] | 22,329,503 | 54.9/5.5 | 34.2/4.8 | 145 | 5 |
|
