Fig. 5

Experiment protocol of asthma model and expression of Moesin in lung tissue of acrolein-treated and sham-treated mice. a. OVA/OVA were sensitized intraperitoneal injection (I.P) at day 0, 14 to BALB/c. All mice received intranasal treatment (I.N) at day 21 to 23 with or without acrolein 1 h before OVA challenge. b and c. acrolein-treated mice increased cell differentials and airway hyperresponsiveness (AHR). d. Western blot analysis of lung cell extracts from sham- or acrolein-treated mice. Moesin expression in lung cells from OVA plus acrolein-treated mice was higher than in lung cells from sham-treated mice. e. lung tissues from acrolein-treated and sham-treated mice were incubated with biotinylated anti-goat Moesin antibody (1100 dilution). Moesin was detected using an avidin-biotin peroxidase complex kit and staining with 3,3′-diaminobenzidine tetrachloride (Zymed Laboratories Inc.) with hematoxylin as a counterstain. Moesin protein expression was notably higher in the lung endothelial cells and epithelial cells from acrolein-treated mice than in that from sham-treated mice