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Table 1 List of proteins identified by MALDI-TOF MS analysis – 30 nM 8 h

From: Proteomic identification of moesin upon exposure to acrolein

No.

Protein name

Abbreviation

Accession no.

Amino acid sequence

pI/molecular mass (Da)

Normalized relative intensity

Ratio

p-value

Function

Untreat

 

Treat(8 hr)

Group 1

1

adenylyl cyclase-associated protein

CAP

178,084

R.SALFAQINQGESITHALK.H

51.926/8.07

0.112379

<

0.606937

5.4008

0.001088

Catalyzing reduction

2

Annexin A5

ANXA5

113,960

K.GLGTDEESILTLLTSR.S

35.971/4.94

0.147797

<

1.02077

6.90659

0.000050

Signal transduction

3

14–3-3 protein epsilon isoform

YWHAE

902,787

K.VAGMDVELTVEER.N

29.326/4.63

0.176267

<

1.13002

6.41086

0.003154

Signal transduction

4

phospholipase A2

PLA2

189,953

K.FLIPNASQAESK.V

27.899/4.73

0.43137

<

1.47095

3.40996

0.000393

Protein modification

5

initiation factor 4D

eIF-4D

181,997

K.VHLVGIDIFTGK.K

17.049/5.08

0.225855

<

0.92464

4.09396

2.5716E-8

Signal transduction

6

fibrin beta

 

223,002

K.HQLYIDETVNSNIPTNLR.V

51.358/7.95

0.167372

<

1.21898

7.28303

0.001577

Coagulation

Group2

7

Tropomyosin beta chain

TPM2

136,090

R.KLVILEGELER.S

32.945/4.66

0.202888

<

0.929909

4.58336

0.000002

Cytoskeleton

8

vimentin

VIM

340,219

R.ETNLDSLPLVDTHSKR.T

53.738/5.03

0.0930222

<

0.363739

3.91024

0.000004

Cytoskeleton

Group 3

9

glyceraldehyde-3-phosphate dehydrogenase

GAPDH

31,645

K.LISWYDNEFGYSNR.V

36.202/8.26

0.712529

>

0.0703642

0.09875

0.000019

Catalyzing reduction

10

Glutathione S-transferase P

GST-Ï€

121,746

R.TLGLYGKDQQEAALVD

MVNDGVEDLR.C

23.569/5.43

0.371254

>

0.0795455

0.21426

0.000011

Antioxidation

Group 4

11

Glutathione S-transferase P

GST-Ï€

121,746

R.TLGLYGKDQQEAALVD

MVNDGVEDLR.C

23.569/5.43

0.342932

>

0.102187

0.29798

0.000009

Antioxidation

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