Proteomic identification of moesin upon exposure to acrolein

Table 3 List of proteins identified by MALDI-TOF MS analysis – 300 nM 8 h

No.	Protein name	Abbreviation	Accession no.	Amino acid sequence	pI/molecular mass (Da)	Normalized relative intensity			Ratio	p-value	Function
No.	Protein name	Abbreviation	Accession no.	Amino acid sequence	pI/molecular mass (Da)	Untreat		Treat(8 h)	Ratio	p-value	Function
Group 1
1	Annexin A1	ANXA1	34,388	K.GVDEATIIDILTKR.N	6.57 /38918	0.06388	<	0.34043	5.32922	0.000218	Signal transduction
2	71 Kd heat shock cognate protein	HSP7C	32,467	K.SFYPEEVSSMVLTK.M	5.37 /71082	0.10343	<	0.525932	5.08493	0.000253	Protein translocation
3	Tropomyosin 4	TPM4	12,803,959	R.KIQALQQQADEAEDR AQGLQR.E	4.67/28667	0.15968	<	0.785374	4.91843	0.000554	Cytoskeleton
4	71 Kd heat shock cognate protein	HSP7C	32,467	K.SFYPEEVSSMVLTK.M	5.37/71082	0.109243	<	0.455133	4.16623	1.1276E-7	Protein translocation
5	14–3-3 protein epsilon isoform	YWHAE	902,787	R.VTIASLPR.N	4.63/29326	0.138728	<	0.536942	3.87054	0.000502	Signal transduction
6	Annexin A5	ANXA5	37,637	R.YLAEFATGNDR.K	4.94/35971	0.06177	<	0.228443	3.6983	8.0228E-7	Signal transduction
7	Annexin A5	ANXA5	37,637	R.LYDAYELK.H	4.94/35971	0.116321	<	0.325831	2.80113	0.000015	Signal transduction
Group 2
8	moesin, isoform CRA_b		119,625,804	K.GLGTDEESILTLLTSR.S	5.90 /66678	0.184177	<	0.519492	2.82061	0.000595	Cytoskeleton
Group 3
9	Glucose-regulated protein 78 precursor, partial	GRP78	386,758	R.TWNDPSVQQDIK.F	5.03 /72185	0.803415	>	0.232592	0.28950	0.002023	Signal transduction
Group 4
10	glyceraldehyde-3-phosphate dehydrogenase	GAPDH	31,645	R.GALQNIIPASTGAAK.A	8.26 /36202	0.560738	>	0.203281	0.36252	0.000884	Catalyzing reduction

ISSN: 1477-5956