Proteomic identification of moesin upon exposure to acrolein

Table 4 List of proteins identified by MALDI-TOF MS analysis – 300 nM 24 h

No.	Protein name	Abbreviation	Accession no.	Amino acid sequence	pI/molecular mass (Da)	Normalized relative intensity			Ratio	p-value	Function
No.	Protein name	Abbreviation	Accession no.	Amino acid sequence	pI/molecular mass (Da)	Untreat		Treat(24 h)	Ratio	p-value	Function
Group 1
1	Annexin A1	ANXA1	34,388	K.GVDEATIIDILTKR.N	6.57 /38918	0.06388	<	0.230679	3.61114	0.000003	Signal transduction
2	71 Kd heat shock cognate protein	HSP7C	32,467	K.SFYPEEVSSMVLTK.M	5.37 /71082	0.10343	<	0.449127	4.34234	4.8387E-7	Protein translocation
3	Tropomyosin 4	TPM4	12,803,959	R.KIQALQQQADEAEDR AQGLQR.E	4.67/28667	0.15968	<	0.546563	3.42287	7.4033E-7	Cytoskeleton
4	71 Kd heat shock cognate protein	HSP7C	32,467	K.SFYPEEVSSMVLTK.M	5.37/71082	0.109243	<	0.256956	2.35214	0.000002	Protein translocation
5	14–3-3 protein epsilon isoform	YWHAE	902,787	R.VTIASLPR.N	4.63/29326	0.138728	<	0.408691	2.94598	0.000007	Signal transduction
6	Annexin A5	ANXA5	37,637	R.YLAEFATGNDR.K	4.94/35971	0.06177	<	0.188239	3.04744	0.000235	Signal transduction
7	Annexin A5	ANXA5	37,637	R.LYDAYELK.H	4.94/35971	0.116321	<	0.252109	2.16735	0.000010	Signal transduction
Group 2
8	moesin, isoform CRA_b		119,625,804	K.GLGTDEESILTLLTSR.S	5.90 /66678	0.184177	<	0.700791	3.80498	0.000061	Cytoskeleton
Group 3
9	Glucose-regulated protein 78 precursor, partial	GRP78	386,758	R.TWNDPSVQQDIK.F	5.03 /72185	0.803415	<	0.825214	1.02713	0.714026	Signal transduction
Group 4
10	glyceraldehyde-3-phosphate dehydrogenase	GAPDH	31,645	R.GALQNIIPASTGAAK.A	8.26 /36202	0.560738	>	0.158456	0.28258	0.000761	Catalyzing reduction