Fig. 2

Hepatic pathology in high fat fed ApoE^−/− mice. a Clustergram of gene names for the differentially expressed proteins found significantly overrepresented in associated MGI Mammalian Phenotype Ontology [61]. Data are shown for top 20 enriched phenotypes, with both phenotypes and differentially expressed proteins clustered according to the combined statistical score. b Immunohistochemical assay of L-FABP expression in animals fed diets for 12 weeks. c Immunoblotting and quantification of L-FABP expression normalized to COXIV. Data are mean ± SD; n = 3 animals/group. d Liver lipids were quantified by triacylglycerol assay. e Histology shows that liver sections from ApoE^−/− HFD mice exhibit minimal to mild steatosis (arrows) at 12 and 16 weeks. Sections were stained with hematoxylin-eosin; original magnification: 100×