Fig. 5

Serum MG-H1 levels and altered GLO1 expression in NAFLD patients. a Correlation of MG-H1 with body mass index (BMI) in adult NAFLD patients (n = 59). b GLO1 immunohistochemistry in a non-NAFLD control case (panels 1,2,3) and in the biopsy specimen of a NAFLD patient (panels 4,5,6). 1: Low magnification reveals expression of GLO1 in hepatocyte cytoplasm without a particular zonal distribution. 2: Low magnification of a negative control section without the primary antibody. 3: High magnification shows cytoplasmic staining (asterisks) and focal nuclear staining (arrow) in hepatocytes. Not all hepatocytes appear to express GLO1 (black triangle). 4: Low magnification reveals bridging fibrosis and severe steatosis with retention of GLO1 staining in non-steatotic foci (arrows); 5: high magnification shows cytoplasmic staining of GLO1 in non-steatotic hepatocytes (black triangle). A peripheral staining rim is noted around large steatotic droplet, and expression of GLO1 in marginalised cytoplasm cannot be excluded (arrows). 6: High magnification reveals nuclear staining (arrows) of GLO1 in many, but not all (triangles), hepatocyte nuclei