Table 2 Deregulated proteins identified by MALDI-TOF in SKOV-3 cells by effect of EOC ascites (n = 5)
Spot No. | Protein Name | Gene Name | Uniprot ID | NCBI/ SwissProt Accession # | Mw (kDa) | Sequences Matched | Mascot Score | Regulation (Folds) | SD | |
|---|---|---|---|---|---|---|---|---|---|---|
Upregulated proteins | 1 | PDZ domain containing protein 11 | PDZD11 | Q5EBL8 | PDZ11_HUMAN | 16.1 | 7 | 64 | + 2.5 | 1.5 |
2 | GTP-binding nuclear protein Ran | RAN | P62826 | gi|48,734,884 | 24.6 | 9 | 90 | + 2.8 | 0.51 | |
3 | Zinc finger protein 268 | ZNF268 | Q14587 | gi|14,579,579 | 92.6 | 11 | 68 | + 2.5 | 0.62 | |
4 | Succinyl-CoA:3-ketoacid-coenzyme A transferase1, OXCT1 | N/A | B7Z609 | gi|363,981,034 | 36.8 | 5 | 72 | + 3.2 | 1.2 | |
5 | Collagen alpha 6(VI) chain | COL6A6 | A6NMZ7 | gi|767,925,744 | 183.4 | 10 | 68 | + 2.8 | 0.05 | |
6 | Actin B | ACTB | P60709 | gi|15,277,503 | 40.5 | 9 | 103 | + 2.4 | 0.78 | |
7 | Keratin, type II cytoskeletal 8 | KRT8 | P05787 | gi|119,617,058 | 53.4 | 11 | 75 | + 2.3 | 0.83 | |
8 | Synaptotagmin-like protein 3 | SYTL3 | Q4VX76 | SYTL3_HUMAN | 70 | 8 | 64 | + 2.5 | 0.52 | |
9 | Vimentin | VIM | P08670 | gi|47,115,317 | 53.6 | 9 | 79 | + 2.7 | 0.12 | |
Downregulated proteins | 10 | Human Leucocytic Antigen I | HLA-I | P01892 | gi|326,416,438 | 21.7 | 6 | 79 | - 2.3 | 0.11 |
11 | Purine nucleoside phosphorylase | PNP | P00491 | gi|37,926,571 | 32.2 | 7 | 69 | - 2.1 | 0.38 | |
12 | Prelamin-A/C | LMNA | P02545 | gi|767,909,266 | 61.6 | 9 | 69 | - 2.4 | 0.76 | |
13 | Actin, cytoplasmic 2 | ACTG1 | P63261 | gi|4,501,887 | 42.1 | 13 | 97 | - 2.2 | 0.99 | |
14 | Heterogeneous nuclear ribonucleoprotein H1 | HNRNPH1 | P31943 | gi|767,938,360 | 50.5 | 7 | 75 | - 2.5 | 1.0 | |
15 | Heat shock protein beta-1 | HSPB1 | P04792 | gi|662,841 | 22.4 | 7 | 91 | - 2.9 | 0.88 | |
16 | ADP ribosylation factor | ARF1 | P84077 | gi|545,719,724 | 44.6 | 8 | 69 | - 2.4 | 0.09 | |
17 | Lon protease mitochondrial | LONP1 | P36776 | gi|414,046 | 95.5 | 10 | 87 | - 3.0 | 1.5 | |
18 | Peroxiredoxin-2 | PRDX2 | P32119 | gi|32,189,392 | 18.4 | 5 | 85 | - 2.6 | 0.48 | |
19 | Cathepsin D | CTSD | P07339 | gi|672,886,498 | 26.7 | 6 | 77 | - 2.5 | 0.89 | |
20 | Synaptotagmin-1 | SYT1 | P21579 | gi|167,744,962 | 32.5 | 8 | 68 | - 2.5 | 1.2 | |