Fig. 1
From: Profiling the interactome of protein kinase C ζ by proteomics and bioinformatics
The overview of the experimental workflow. PKCζ interacting proteins characterized using CoIP-MS detection. The observed proteins were filtered using the CRAPome database to eliminate the potential false positives. The proteins identified using mass spectrometry were combined with PKCζ interacting proteins identified through literature mining and PPI database search. The complete list of PKCζ interacting proteins were analyzed using the PANTHER database. Furthermore, the interaction between each protein was obtained using STRING PPI database search, and the results were uploaded into Cytoscape for network construction