Effects of the complete replacement of fish oil with linseed oil on growth, fatty acid composition, and protein expression in the Chinese mitten crab (Eriocheir sinensis)

Table 1 Composition and calculated proximate composition of the diet

Ingredients (%)	Diets group
Ingredients (%)	FO	LO
Casein	41	41
Cellulose	4	4
Wheat flour	28.65	28.65
Carboxymethylcellulose (CMC)	4	4
Yeast extract	5	5
Lysine	0.15	0.15
Glycine	0.5	0.5
Vitamin C (99.7%)	0.5	0.5
Vitamin E (97%)	0.1	0.1
Phospholipid (99%)	3	3
Cholesterol	0.5	0.5
Inositol	0.6	0.6
Choline chloride (50%)	1	1
Mineral premix^a	3	3
Vitamin premix^b	2	2
Fish oil	6	0
Linseed oil	0	6
Proximate composition (percentage of dry weight)
Crude protein	39.26	39.44
Crude lipid	9.75	9.47
Ash	5.68	5.72

^aMineral premix: 1 kg diet contained Ca(H₂PO₄)₂, 10 g; MgSO₄·7H₂O, 2.4 g; KCl, 4.5 g; NaCl, 2.1 g; FeSO₄·H₂O, 155 mg; CuSO₄·5H₂O, 40 mg; ZnSO₄·H₂O, 80 mg; MnSO₄·H₂O, 30 mg; KI, 11.7 mg; CoCl₂·6H₂O, 4.8 mg; Na₂SeO₃, 2.4 mg
^bVitamin premix: 1 kg diet contained vitamin A, 10000 IU; vitamin D, 2500 IU; vitamin K, 64 mg; thiamin, 60 mg; riboflavin, 250 mg; pyridoxine, 60 mg; calcium pantothenate, 240 mg; niacin, 60 mg; folic acid, 12 mg; biotin, 50 mg; cyanocobalamin, 4 mg

ISSN: 1477-5956