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Table 3 Protein carbonylation profile by comparison of No treatment and FA treatment of HepG2

From: Hepatic protein Carbonylation profiles induced by lipid accumulation and oxidative stress for investigating cellular response to non-alcoholic fatty liver disease in vitro

Spot No. Protein ID Protein name 2DE Prediction Mascot score Sequence coverage Log2 Ratio*
MW(kDa) pI MW(kDa) pI
16 P00338 L-lactate dehydrogenase A chain isoform 3 39 8.6 36.6 8.44 1732 80.1 0.00
34 P04057 Fructose-bisphosphate aldolase A isoform 1 48 7.7 13.2 8.52 2058 86.5 − 1.36
35 P78330 Phosphoserine phosphatase 29 5.5 25 5.51 1246 84 0.00
36 P35270 Sepiapterin reductase 28 8.7 28.0 8.2 820 67 −3.47
37 P11021 78 kDa glucose-regulated protein 86 4.3 72.3 5.0 277 12 −1.65
38 P68371 Tubulin beta-4B chain 66 3.6 49.8 4.7 2177 83.4 −1.43
39 Q9Y3F4 Serine-threonine kinase receptor-associated protein 46 4.1 38.4 4.9 56 5 −2.06
40 P55735 Protein SEC13 homolog 43 4.4 35.5 5.2 88 19 −1.03
41 Q13733 Sodium/potassium-transporting ATPase subunit alpha-4 40 4.9 114.1 6.2 29 2 0.00
42 Q15181 Inorganic pyrophosphatase 38 5.6 32.6 5.5 309 35 −2.19
43 Q9HC38 Glyoxalase domain-containing protein 4 36 5.2 34.7 5.4 31 4 0.00
44 P08758 Annexin A5 36 4.0 35.9 4.9 1419 58 −1.29
45 P12004 Proliferating cell nuclear antigen 37 3.4 28.7 4.5 406 62 −1.29
46 Q15691 Microtubule-associated protein RP/EB family member 1 35 4.3 29.9 5.0 60 27 −1.74
47 Q99943 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha 30 4.4 31.7 9.48 38 3 −1.43
48 P35232 Prohibitin 31 5.6 29.8 5.5 1199 59 −1.84
49 P29401 Transketolase 74 8.5 67.8 7.5 791 41 −3.47
50 Q9UL46 Proteasome activator complex subunit 2 32 5.1 27.4 5.5 723 74 0.00
51 P25705 ATP synthase subunit alpha, mitochondrial 31 9.4 59.7 5.1 1222 42.7 −1.58
  1. * Log2 Ratio refers to Log2 ratio of spot intensity from FA treatment to No treatment