Table 3 Protein carbonylation profile by comparison of No treatment and FA treatment of HepG2
Spot No. | Protein ID | Protein name | 2DE | Prediction | Mascot score | Sequence coverage | Log2 Ratio* | ||
|---|---|---|---|---|---|---|---|---|---|
MW(kDa) | pI | MW(kDa) | pI | ||||||
16 | P00338 | L-lactate dehydrogenase A chain isoform 3 | 39 | 8.6 | 36.6 | 8.44 | 1732 | 80.1 | 0.00 |
34 | P04057 | Fructose-bisphosphate aldolase A isoform 1 | 48 | 7.7 | 13.2 | 8.52 | 2058 | 86.5 | − 1.36 |
35 | P78330 | Phosphoserine phosphatase | 29 | 5.5 | 25 | 5.51 | 1246 | 84 | 0.00 |
36 | P35270 | Sepiapterin reductase | 28 | 8.7 | 28.0 | 8.2 | 820 | 67 | −3.47 |
37 | P11021 | 78 kDa glucose-regulated protein | 86 | 4.3 | 72.3 | 5.0 | 277 | 12 | −1.65 |
38 | P68371 | Tubulin beta-4B chain | 66 | 3.6 | 49.8 | 4.7 | 2177 | 83.4 | −1.43 |
39 | Q9Y3F4 | Serine-threonine kinase receptor-associated protein | 46 | 4.1 | 38.4 | 4.9 | 56 | 5 | −2.06 |
40 | P55735 | Protein SEC13 homolog | 43 | 4.4 | 35.5 | 5.2 | 88 | 19 | −1.03 |
41 | Q13733 | Sodium/potassium-transporting ATPase subunit alpha-4 | 40 | 4.9 | 114.1 | 6.2 | 29 | 2 | 0.00 |
42 | Q15181 | Inorganic pyrophosphatase | 38 | 5.6 | 32.6 | 5.5 | 309 | 35 | −2.19 |
43 | Q9HC38 | Glyoxalase domain-containing protein 4 | 36 | 5.2 | 34.7 | 5.4 | 31 | 4 | 0.00 |
44 | P08758 | Annexin A5 | 36 | 4.0 | 35.9 | 4.9 | 1419 | 58 | −1.29 |
45 | P12004 | Proliferating cell nuclear antigen | 37 | 3.4 | 28.7 | 4.5 | 406 | 62 | −1.29 |
46 | Q15691 | Microtubule-associated protein RP/EB family member 1 | 35 | 4.3 | 29.9 | 5.0 | 60 | 27 | −1.74 |
47 | Q99943 | 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha | 30 | 4.4 | 31.7 | 9.48 | 38 | 3 | −1.43 |
48 | P35232 | Prohibitin | 31 | 5.6 | 29.8 | 5.5 | 1199 | 59 | −1.84 |
49 | P29401 | Transketolase | 74 | 8.5 | 67.8 | 7.5 | 791 | 41 | −3.47 |
50 | Q9UL46 | Proteasome activator complex subunit 2 | 32 | 5.1 | 27.4 | 5.5 | 723 | 74 | 0.00 |
51 | P25705 | ATP synthase subunit alpha, mitochondrial | 31 | 9.4 | 59.7 | 5.1 | 1222 | 42.7 | −1.58 |