Table 4 Protein carbonylation profile by comparison of No treatment and FA/MND treatment of HepG2
Spot No. | Protein ID | Protein name | 2DE | Prediction | Mascot score | Sequence coverage | Log2 Ratio* | ||
|---|---|---|---|---|---|---|---|---|---|
MW(kDa) | pI | MW(kDa) | pI | ||||||
16 | P00338 | L-lactate dehydrogenase A chain isoform 3 | 39 | 8.6 | 36.6 | 8.44 | 1732 | 80.1 | 0.00 |
34 | P04057 | Fructose-bisphosphate aldolase A isoform 1 | 48 | 7.7 | 13.2 | 8.52 | 2058 | 86.5 | −2.00 |
35 | P78330 | Phosphoserine phosphatase | 29 | 5.5 | 25 | 5.51 | 1246 | 84 | −2.40 |
36 | P35270 | Sepiapterin reductase | 28 | 8.7 | 28.0 | 8.2 | 820 | 67 | −2.64 |
38 | P68371 | Tubulin beta-4B chain | 66 | 3.6 | 49.8 | 4.7 | 2177 | 83.4 | −1.03 |
39 | Q9Y3F4 | Serine-threonine kinase receptor-associated protein | 46 | 4.1 | 38.4 | 4.9 | 56 | 5 | −1.40 |
40 | P55735 | Protein SEC13 homolog | 43 | 4.4 | 35.5 | 5.2 | 88 | 19 | 0.00 |
41 | Q13733 | Sodium/potassium-transporting ATPase subunit alpha-4 | 40 | 4.9 | 114.1 | 6.2 | 29 | 2 | 0.00 |
42 | Q15181 | Inorganic pyrophosphatase | 38 | 5.6 | 32.6 | 5.5 | 309 | 35 | −2.64 |
43 | Q9HC38 | Glyoxalase domain-containing protein 4 | 36 | 5.2 | 34.7 | 5.4 | 31 | 4 | 0.00 |
46 | Q15691 | Microtubule-associated protein RP/EB family member 1 | 35 | 4.3 | 29.9 | 5.0 | 60 | 27 | −2.06 |
50 | Q9UL46 | Proteasome activator complex subunit 2 | 32 | 5.1 | 27.4 | 5.5 | 723 | 74 | 0.00 |
51 | P25705 | ATP synthase subunit alpha, mitochondrial | 31 | 9.4 | 59.7 | 5.1 | 1222 | 42.7 | −1.89 |
52 | A0A024QZX5 | Serpin B6 isoform B | 47 | 4.9 | 43.0 | 5.1 | 1876 | 82.4 | −1.58 |
53 | P11021 | 78 kDa glucose-regulated protein | 85 | 4.1 | 72.3 | 5.0 | 2083 | 56 | −1.06 |
54 | P10809 | 60 kDa heat shock protein, mitochondrial | 66 | 4.8 | 61.0 | 5.7 | 329 | 26 | 0.00 |
55 | Q5T6V5 | UPF0553 protein C9orf64 | 43 | 5.5 | 39.0 | 5.6 | 191 | 11 | −3.64 |
56 | P09661 | U2 small nuclear ribonucleoprotein A’ | 33 | 9.0 | 28.4 | 8.7 | 131 | 4 | −1.69 |
57 | P54819 | Adenylate kinase 2, mitochondrial isoform a | 30 | 8.6 | 26.4 | 7.67 | 1248 | 40 | −1.25 |
58 | P04075 | Fructose-bisphosphate aldolase A | 27 | 8.9 | 39.4 | 8.3 | 47 | 6 | −2.64 |