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Table 4 Protein carbonylation profile by comparison of No treatment and FA/MND treatment of HepG2

From: Hepatic protein Carbonylation profiles induced by lipid accumulation and oxidative stress for investigating cellular response to non-alcoholic fatty liver disease in vitro

Spot No. Protein ID Protein name 2DE Prediction Mascot score Sequence coverage Log2 Ratio*
MW(kDa) pI MW(kDa) pI
16 P00338 L-lactate dehydrogenase A chain isoform 3 39 8.6 36.6 8.44 1732 80.1 0.00
34 P04057 Fructose-bisphosphate aldolase A isoform 1 48 7.7 13.2 8.52 2058 86.5 −2.00
35 P78330 Phosphoserine phosphatase 29 5.5 25 5.51 1246 84 −2.40
36 P35270 Sepiapterin reductase 28 8.7 28.0 8.2 820 67 −2.64
38 P68371 Tubulin beta-4B chain 66 3.6 49.8 4.7 2177 83.4 −1.03
39 Q9Y3F4 Serine-threonine kinase receptor-associated protein 46 4.1 38.4 4.9 56 5 −1.40
40 P55735 Protein SEC13 homolog 43 4.4 35.5 5.2 88 19 0.00
41 Q13733 Sodium/potassium-transporting ATPase subunit alpha-4 40 4.9 114.1 6.2 29 2 0.00
42 Q15181 Inorganic pyrophosphatase 38 5.6 32.6 5.5 309 35 −2.64
43 Q9HC38 Glyoxalase domain-containing protein 4 36 5.2 34.7 5.4 31 4 0.00
46 Q15691 Microtubule-associated protein RP/EB family member 1 35 4.3 29.9 5.0 60 27 −2.06
50 Q9UL46 Proteasome activator complex subunit 2 32 5.1 27.4 5.5 723 74 0.00
51 P25705 ATP synthase subunit alpha, mitochondrial 31 9.4 59.7 5.1 1222 42.7 −1.89
52 A0A024QZX5 Serpin B6 isoform B 47 4.9 43.0 5.1 1876 82.4 −1.58
53 P11021 78 kDa glucose-regulated protein 85 4.1 72.3 5.0 2083 56 −1.06
54 P10809 60 kDa heat shock protein, mitochondrial 66 4.8 61.0 5.7 329 26 0.00
55 Q5T6V5 UPF0553 protein C9orf64 43 5.5 39.0 5.6 191 11 −3.64
56 P09661 U2 small nuclear ribonucleoprotein A’ 33 9.0 28.4 8.7 131 4 −1.69
57 P54819 Adenylate kinase 2, mitochondrial isoform a 30 8.6 26.4 7.67 1248 40 −1.25
58 P04075 Fructose-bisphosphate aldolase A 27 8.9 39.4 8.3 47 6 −2.64
  1. *Log2 Ratio refers to Log2 ratio of spot intensity from FA/MND treatment to No treatment