Table 1 List of B. fibrisolvens 3071 proteins identified and confirmed by nLC/MS as significantly different. The spots were excised from the 2-D electrophoresis gels comparing glucose/xylan (I), glucose/xylan + glucose (II), glucose/xylose (III), xylan/xylan + glucose (IV), xylan/xylose (V), xylan + glucose/xylose (VI)
Substrate comparison | Spot number | Accession number | Protein | Total number of peptides | Fold change | MW | pI |
|---|---|---|---|---|---|---|---|
I | 1a | SHI27642.1 | diaminopimelate dehydrogenase | 3 | 3.99 ↑g1 | 36.2 | 5.5 |
II | 2b | SHH89614.1 | glucose-6-phosphate isomerase | 18 | 0.61 ↑x + g3 | 56.9 | 5.1 |
3b | SHI23166.1 | N-acetylmuramoyl-L-alanine amidase | 6 | 0.44 ↑x + g3 | 38.9 | 5.2 | |
4b | SHH52657.1 | 1-phosphofructokinase | 9 | 0.61 ↑x + g3 | 32.6 | 4.7 | |
5b | SER24636.1 | phosphoglycerate kinase | 6 | 2.02 ↑g1 | 43.8 | 5.2 | |
III | 6c | BAD51424.1 | beta-hydroxybutyryl-CoA dehydrogenase | 7 | 2.29 ↑g1 | 31.5 | 5.7 |
IV | 7d | SHI29611.1 | fructose-bisphosphate aldolase, class II | 19 | 0.61 ↑x + g3 | 33.9 | 5.2 |
V | 8e | SHI15246.1 | elongation factor Ts | 13 | 0.81 ↑xyl4 | 34.6 | 4.8 |
9e | SHI83999.1 | glyceraldehyde 3-phosphate dehydrogenase | 8 | 0.88 ↑xyl4 | 36.9 | 5.8 | |
10e | SHH82532.1 | 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase | 2 | 0.69 ↑x2 | 38.5 | 6.0 | |
11e | SHI15246.1 | elongation factor Ts | 13 | 0.93 ↑x2 | 34.6 | 4.8 | |
VI | 12f | SER24636.1 | phosphoglycerate kinase | 2 | 2.22 ↑x + g3 | 46.8 | 5.2 |
13f | SER75936.1 | fructose-bisphosphate aldolase, class II | 7 | 1.04 ↑x + g3 | 33.9 | 5.2 | |
14f | SHI15246.1 | elongation factor Ts | 2 | 0.62 ↑xyl4 | 34.6 | 4.8 |