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Table 1 Advantages and Disadvantages of proteomic digestion techniques

From: Exploring the key communicator role of exosomes in cancer microenvironment through proteomics

Digestion Method Advantages Disadvantages
In-gel Digestion Reproducible, Cost effective, Removal of mass spectrometry incompatible detergents (SDS, Triton etc.) and contaminants, Wide cover range Time consuming, Inacceptable for extremely acidic or basic and high or low molecular weight proteins and membrane proteins
In-sol Digestion Require less time Inacceptable for low resolubilization proteins
FASP Acceptable for membrane proteins, Removal of mass spectrometry incompatible detergents (SDS, Triton etc.) Loss of proteins , Bad repoducibility, Require large amount of protein sample (> 50ug)