Table 1 Advantages and Disadvantages of proteomic digestion techniques
From: Exploring the key communicator role of exosomes in cancer microenvironment through proteomics
Digestion Method | Advantages | Disadvantages |
|---|---|---|
In-gel Digestion | Reproducible, Cost effective, Removal of mass spectrometry incompatible detergents (SDS, Triton etc.) and contaminants, Wide cover range | Time consuming, Inacceptable for extremely acidic or basic and high or low molecular weight proteins and membrane proteins |
In-sol Digestion | Require less time | Inacceptable for low resolubilization proteins |
FASP | Acceptable for membrane proteins, Removal of mass spectrometry incompatible detergents (SDS, Triton etc.) | Loss of proteins , Bad repoducibility, Require large amount of protein sample (> 50ug) |