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Table 2. Summarization of used techniques for cancer-derived exosomes isolation and exosomal protein digestion methods

From: Exploring the key communicator role of exosomes in cancer microenvironment through proteomics

Origin of Exosome Isolation Method Digestion Method Reference
Cell Ultracentrifuge In-Gel Digestion [23,24,25,26,27,28,29,30]
In-Sol Digestion [26, 31, 32]
Precipitation reagent In-Gel Digestion [33,34,35,36,37]
FASP [38, 39]
Combined method In-Gel Digestion [28, 35, 36, 40,41,42,43,44,45,46,47,48,49,50,51]
In-Sol Digestion [52,53,54]
FASP [51, 55,56,57,58,59]
Plasma/ Serum Ultracentrifuge In-Sol Digestion [60]
FASP [56]
Combined method In-Gel Digestion [43]
In-Sol Digestion [44, 52]
FASP [51, 57, 58]