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Table 2. Summarization of used techniques for cancer-derived exosomes isolation and exosomal protein digestion methods

From: Exploring the key communicator role of exosomes in cancer microenvironment through proteomics

Origin of Exosome

Isolation Method

Digestion Method

Reference

Cell

Ultracentrifuge

In-Gel Digestion

[23,24,25,26,27,28,29,30]

In-Sol Digestion

[26, 31, 32]

Precipitation reagent

In-Gel Digestion

[33,34,35,36,37]

FASP

[38, 39]

Combined method

In-Gel Digestion

[28, 35, 36, 40,41,42,43,44,45,46,47,48,49,50,51]

In-Sol Digestion

[52,53,54]

FASP

[51, 55,56,57,58,59]

Plasma/ Serum

Ultracentrifuge

In-Sol Digestion

[60]

FASP

[56]

Combined method

In-Gel Digestion

[43]

In-Sol Digestion

[44, 52]

FASP

[51, 57, 58]