Table 1 Proteomic studies in the case of FSGS, MGN and IgAN
CKD type | Sample Type | Method | Putative Biomarkers | Ref. |
|---|---|---|---|---|
MGN | Tissue | MALDI-MS imaging | sonic Hedgehog (SHH), α-smooth muscle actin (α-SMA) | (Smith et al., 2019) |
Urine | tandem mass tag (TMT), nano-scale LC-MS/MS | Alpha-1-antitrypsin, Serotransferrin, Ceruloplasmin, Serum albumin, Carbonic anhydrase 1, Transthyretin, Haptoglobin, Leucine-rich alpha-2-glycoprotein, Alpha-1B-glycoprotein, Afamin | (Pang et al., 2018) | |
Serum, Tissue | 2DE, semidry blotting, Mass | α-enolase, superoxide dismutase 2, vimentin, aldolase reductase, glycine-tRNA ligase, elongation factor 2 | (Bruschi et al., 2015) | |
Urine | 2DE, MALDI-TOF-MS | progesterone-induced blocking factor 1 isoform a, ribosomal protein L5, vimentin, tropomyosin isoform 6, α-1-antitrypsin, serotransferrin precursor, Ba1–647, haptoglobin precursor, preprohaptoglobin, serum albumin precursor, CTP:phosphocholine cytidylyl transferase, anti-idiotype immunoglobulin M light chain, vascular endothelial growth factor A splice variant VEGF 102 | (Ngai et al., 2006) | |
Serum | SDS-PAGE | 29 KD, 5 KD | (Pant et al., 2016) | |
Tissue | iTRAQ, MALDI-TOF-TOF-MS | C1RL, TUBB, CD74, CORO1A, B2M, KRT1, ARHGDIB, HLA-DRB1, HLA-A, HLA-DRB1, IGHG1, KV102, ERAP1, MYLPF, GBP1, HLA-B, FTH1, VCAM1, C4BPA, HSPD1, C1QBP | (Sui et al., 2015) | |
Interstitial Fluid, Tissue, Urine, Serum, Podocyte Cell Lines | ESI-Q-TOF-MS, immunofluorescence | PDLI5, LDB3 | (Ligabue et al., 2013) | |
Urinary Microvesicles, Tissue | iTRAQ labeling and LC-MS/MS, immunofluorescence | LIMP2, TPP1, AMBP, PDZK1P1, DCHS2, MGAM, SLC9A3R1, ATAD5, HYDIN, MYO15A, C7, MUC1, SDCBP, GPRC5B, ATG2B, RAC1, EHMT1, RHOC, KMT2B, EPS8, EVC2, SLC44A2, SLC12A3, MYO9B, CCDC81 | (Rood et al., 2015) | |
Tissue | MALDI-MS imaging | serine/threonine protein kinase MRCK gamma, PLA2R, IgG4 | (Smith et al., 2017) | |
IgAN | Tissue | immunostaining | CAP1, PRCP, SHC1 | (Krochmal et al., 2017) |
Urine | IEF/LC-MS/MS | AFM, ALB, APOA1, AZGP1, A2M, CA1, CP, C3, C4A, FN1, F2, GC, HP, IGFBP7, PSAP, SERPINA1, SERPINA3 | (Mucha et al., 2014) | |
Urine | nanoscale LC-high resolution tandem MS | transferrin, α1-antitrypsin, albumin fragments, fibulin-5, prasoposin, YIP1 family member 3, osteopontin | (Mohammadi Majd et al., 2018) | |
Urine | 2D-LC-MS/MS, iTRAQ, Western blot | ICAM1, TIMP1, SERPINC1, ADIPOQ | (Guo et al., 2018) | |
Urine | LC-MALDI-TOF/MS, proteins arrays, immunomagnetic isolation | IgA-uromodulin complex, α1-antitrypsin, galactose-deficient IgA1 | (Neprasova et al., 2016) | |
Tissue | MALDI-MS imaging | m/z of 4963, 5072 | (Smith et al., 2016) | |
Urine | nano-LC-MS/MS | CD44, glycoprotein 2, vasorin, protocadherin, epidermal growth factor, CLM9, dipeptidyl peptidase IV, NHL repeat-containing protein 3, utreoglobin, SLAM family member 5 | (Samavat et al., 2015) | |
Urine | SELDI-TOF-MS, MALDI-TOF-MS | perlecan laminin G-like peptide, IgK light chains | (Rocchetti et al., 2013) | |
Urine, Serum, Plasma | magnetic bead technology, MALDI-TOF-MS | uromodulin, bradykinin and α-1-antitrypsin urine m/z:1769, 1898, 1913, 1945, 2491, 2756, 2977, 3004, 3389, 4752 blood m/z:2953, 5337, 9287, 9289 | (Graterol et al., 2013) | |
Urine | MALDI-TOF-MS | m/z: 2740, 3038, 2747, 3032, 3235, 2095, 3328, 6174, 6189, 2754 | (He et al., 2012) | |
Urine | 2DE, nano-HPLC-ESI-MS/MS | kininogen, inter-α-trypsin-inhibitor heavy chain 4, tranthyretin | (Rocchetti et al., 2008) | |
Urine | 2DE, MALDI-TOF-MS | A panel of 59 protein markers | (Park et al., 2006) | |
Urine | 2D-DIGE, LC-MS/MS | α1-microglobulin, transferrin, albumin, retinol-binding protein 4, β-globin, carbonic anhydrase I, cystatin C, α2-glycoprotein 1 | (Yokota et al., 2007) | |
Urinary Exosomes | LC-MS/MS | aminopeptidase N, α-1-antitrypsin, vasorin precursor, ceruloplasmin | (Moon et al., 2011) | |
Urine | 2DE, MALDI-TOF-TOF/MS, western blot | α-1-antitrypsin, albumin fragments, α-1-β-glycoprotein, laminin G-like fragment of endorepellin | (Surin et al., 2013) | |
Urine | CE-ESI-TOF-MS | A panel of polypeptides molecular weights | (Haubitz et al., 2005) | |
| Â | Urine | nanoflow LC-MS/MS, GeLC-MS/MS | afamin, leucine-rich alpha-2-glycoprotein, ceruloplasmin, alpha-1-microgolbulin, hemopexin, apolipoprotein A-I, complement C3, vitamin D-binding protein, beta-2-microglobulin, retinol-binding protein 4 | (Kalantari et al. 2013) |
FSGS | Urine | nano-flow LC-MS/MS | RNAS2, CD59, PTGDS, B2MG, AMBP, SULF2, CBG, AFAM, MXRA8, CO6A1, ACTG, HPT | (S. Kalantari et al., 2014) |
Urine | LC-MS/MS, Western blot | serum albumin, serotransferrin, α-1-1antiproteinase, afamin, ceruloplasmin, plasminogen, AMBP | (Zhao et al., 2014) | |
Urine | magnetic bead-based technology, MALDI-TOF/MS | Uromodulin, α-1-antitrypsin fragment | (Perez et al., 2014) | |
Urine | nano-LC-MS/MS | apolipoprotein-A1, matrix-remodeling protein 8 | (Shiva Kalantari et al., 2014) | |
Urine | 2D-DIGE, MALDI-TOF MS | α-1-antitrypsin, transferrin, 39S ribosomal protein L17, histatin-3, calretinin | (Perez et al., 2017) | |
Urine | 2DE, MALDI-TOF/MS | glutathione S-transferase, collagen IV fragment, E-cadherin | (Shui et al., 2008) | |
Urine | 2DE, tandem-MS | UBA52, CD74, SKP1, CXADR, S100A13, SYNE1, PVALB, FRY, GSTA3, CROCC, LRBA, DAB2 | (Wang et al., 2017) | |
Serum | SDS-PAGE | 29 KD, 5 KD | (Pant et al., 2016) | |
Tissue | MALDI-MS imaging | m/z of 4025, 4048 | (Smith et al., 2016) | |
Urine | nano-flow LC-MS/MS | CD59, CD44, IBP7, DPEP1, Robo4 | (Nafar et al., 2014) |