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Table 1 Proteomic studies in the case of FSGS, MGN and IgAN

From: Chronic kidney disease: a review of proteomic and metabolomic approaches to membranous glomerulonephritis, focal segmental glomerulosclerosis, and IgA nephropathy biomarkers

CKD typeSample TypeMethodPutative BiomarkersRef.
MGNTissueMALDI-MS imagingsonic Hedgehog (SHH), α-smooth muscle actin (α-SMA)(Smith et al., 2019)
Urinetandem mass tag (TMT), nano-scale LC-MS/MSAlpha-1-antitrypsin, Serotransferrin, Ceruloplasmin, Serum albumin, Carbonic anhydrase 1, Transthyretin, Haptoglobin, Leucine-rich alpha-2-glycoprotein, Alpha-1B-glycoprotein, Afamin(Pang et al., 2018)
Serum, Tissue2DE, semidry blotting, Massα-enolase, superoxide dismutase 2, vimentin, aldolase reductase, glycine-tRNA ligase, elongation factor 2(Bruschi et al., 2015)
Urine2DE, MALDI-TOF-MSprogesterone-induced blocking factor 1 isoform a, ribosomal protein L5, vimentin, tropomyosin isoform 6, α-1-antitrypsin, serotransferrin precursor, Ba1–647, haptoglobin precursor, preprohaptoglobin, serum albumin precursor, CTP:phosphocholine cytidylyl transferase, anti-idiotype immunoglobulin M light chain, vascular endothelial growth factor A splice variant VEGF 102(Ngai et al., 2006)
SerumSDS-PAGE29 KD, 5 KD(Pant et al., 2016)
TissueiTRAQ, MALDI-TOF-TOF-MSC1RL, TUBB, CD74, CORO1A, B2M, KRT1, ARHGDIB, HLA-DRB1, HLA-A, HLA-DRB1, IGHG1, KV102, ERAP1, MYLPF, GBP1, HLA-B, FTH1, VCAM1, C4BPA, HSPD1, C1QBP(Sui et al., 2015)
Interstitial Fluid, Tissue, Urine, Serum, Podocyte Cell LinesESI-Q-TOF-MS, immunofluorescencePDLI5, LDB3(Ligabue et al., 2013)
Urinary Microvesicles, TissueiTRAQ labeling and LC-MS/MS, immunofluorescenceLIMP2, TPP1, AMBP, PDZK1P1, DCHS2, MGAM, SLC9A3R1, ATAD5, HYDIN, MYO15A, C7, MUC1, SDCBP, GPRC5B, ATG2B, RAC1, EHMT1, RHOC, KMT2B, EPS8, EVC2, SLC44A2, SLC12A3, MYO9B, CCDC81(Rood et al., 2015)
TissueMALDI-MS imagingserine/threonine protein kinase MRCK gamma, PLA2R, IgG4(Smith et al., 2017)
IgANTissueimmunostainingCAP1, PRCP, SHC1(Krochmal et al., 2017)
UrineIEF/LC-MS/MSAFM, ALB, APOA1, AZGP1, A2M, CA1, CP, C3, C4A, FN1, F2, GC, HP, IGFBP7, PSAP, SERPINA1, SERPINA3(Mucha et al., 2014)
Urinenanoscale LC-high resolution tandem MStransferrin, α1-antitrypsin, albumin fragments, fibulin-5, prasoposin, YIP1 family member 3, osteopontin(Mohammadi Majd et al., 2018)
Urine2D-LC-MS/MS, iTRAQ, Western blotICAM1, TIMP1, SERPINC1, ADIPOQ(Guo et al., 2018)
UrineLC-MALDI-TOF/MS, proteins arrays, immunomagnetic isolationIgA-uromodulin complex, α1-antitrypsin, galactose-deficient IgA1(Neprasova et al., 2016)
TissueMALDI-MS imagingm/z of 4963, 5072(Smith et al., 2016)
Urinenano-LC-MS/MSCD44, glycoprotein 2, vasorin, protocadherin, epidermal growth factor, CLM9, dipeptidyl peptidase IV, NHL repeat-containing protein 3, utreoglobin, SLAM family member 5(Samavat et al., 2015)
UrineSELDI-TOF-MS, MALDI-TOF-MSperlecan laminin G-like peptide, IgK light chains(Rocchetti et al., 2013)
Urine, Serum, Plasmamagnetic bead technology, MALDI-TOF-MSuromodulin, bradykinin and α-1-antitrypsin urine m/z:1769, 1898, 1913, 1945, 2491, 2756, 2977, 3004, 3389, 4752 blood m/z:2953, 5337, 9287, 9289(Graterol et al., 2013)
UrineMALDI-TOF-MSm/z: 2740, 3038, 2747, 3032, 3235, 2095, 3328, 6174, 6189, 2754(He et al., 2012)
Urine2DE, nano-HPLC-ESI-MS/MSkininogen, inter-α-trypsin-inhibitor heavy chain 4, tranthyretin(Rocchetti et al., 2008)
Urine2DE, MALDI-TOF-MSA panel of 59 protein markers(Park et al., 2006)
Urine2D-DIGE, LC-MS/MSα1-microglobulin, transferrin, albumin, retinol-binding protein 4, β-globin, carbonic anhydrase I, cystatin C, α2-glycoprotein 1(Yokota et al., 2007)
Urinary ExosomesLC-MS/MSaminopeptidase N, α-1-antitrypsin, vasorin precursor, ceruloplasmin(Moon et al., 2011)
Urine2DE, MALDI-TOF-TOF/MS, western blotα-1-antitrypsin, albumin fragments, α-1-β-glycoprotein, laminin G-like fragment of endorepellin(Surin et al., 2013)
UrineCE-ESI-TOF-MSA panel of polypeptides molecular weights(Haubitz et al., 2005)
 Urinenanoflow LC-MS/MS, GeLC-MS/MSafamin, leucine-rich alpha-2-glycoprotein, ceruloplasmin, alpha-1-microgolbulin, hemopexin, apolipoprotein A-I, complement C3, vitamin D-binding protein, beta-2-microglobulin, retinol-binding protein 4(Kalantari et al. 2013)
FSGSUrinenano-flow LC-MS/MSRNAS2, CD59, PTGDS, B2MG, AMBP, SULF2, CBG, AFAM, MXRA8, CO6A1, ACTG, HPT(S. Kalantari et al., 2014)
UrineLC-MS/MS, Western blotserum albumin, serotransferrin, α-1-1antiproteinase, afamin, ceruloplasmin, plasminogen, AMBP(Zhao et al., 2014)
Urinemagnetic bead-based technology, MALDI-TOF/MSUromodulin, α-1-antitrypsin fragment(Perez et al., 2014)
Urinenano-LC-MS/MSapolipoprotein-A1, matrix-remodeling protein 8(Shiva Kalantari et al., 2014)
Urine2D-DIGE, MALDI-TOF MSα-1-antitrypsin, transferrin, 39S ribosomal protein L17, histatin-3, calretinin(Perez et al., 2017)
Urine2DE, MALDI-TOF/MSglutathione S-transferase, collagen IV fragment, E-cadherin(Shui et al., 2008)
Urine2DE, tandem-MSUBA52, CD74, SKP1, CXADR, S100A13, SYNE1, PVALB, FRY, GSTA3, CROCC, LRBA, DAB2(Wang et al., 2017)
SerumSDS-PAGE29 KD, 5 KD(Pant et al., 2016)
TissueMALDI-MS imagingm/z of 4025, 4048(Smith et al., 2016)
Urinenano-flow LC-MS/MSCD59, CD44, IBP7, DPEP1, Robo4(Nafar et al., 2014)
  1. MGN Membranous glomerulonephritis, IgAN Immunoglobulin A nephropathy, FSGS Focal segmental glomerulosclerosis, MCD Minimal change disease, iFSGS idiopathic Focal segmental glomerulosclerosis, iMGN idiopathic Membranous glomerulonephritis, MPG Membranoproliferative glomerulonephritis, DN Diabetic nephropathy, LN Lupus nephritis, TBMN Thin-basement membranous nephropathy