Fig. 4
From: NEK10 interactome and depletion reveal new roles in mitochondria
The essential role of NEK10 for mitochondria morphology. a Representative confocal images of mitochondrial morphology in HeLa cells shRNA for Nek10 (pLKO-sh89 and pLKO-sh90) or control (pLKO-empty) stained with MitoTracker DeepRed for 20 min. Micrographs were acquired from live cells, using 63× oil objective, 1 air unit pinhole, 1024 × 1024 pixels. Scale bars: 10 μm. b Quantitative analysis of mitochondrial morphology is shown. Cells were classified on the basis of their mitochondrial morphology: oval/spheres, short (< 6 μm) or long (> 6 μm) mitochondrial filaments. At least 30 cells/sample were counted. One way ANOVA was used followed by the Bonferroni post hoc test. * = p < 0.05; *** = P < 0.001