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Fig. 6 | Proteome Science

Fig. 6

From: NEK10 interactome and depletion reveal new roles in mitochondria

Fig. 6

The mitochondrial respiratory chain is negatively affected by the depletion of NEK10. a The graphic shows O2 Flow per cells by basal respiration and after treatments (Olygomycin, CCCP and Rotenone) for HeLa pLKO-empty, HeLa pLKO-sh89 and HeLa pLKO-sh90. b Visualization of experimental oxygen consumption rate (OCR) data type nomenclature, adapted from Rose and coworkers [56]. c Measurement of ATP-Linked to Oxygen Consumption Rate (OCR). d Measurement of spare capacity. e Measurement of proton leak. f Measurement of non-mitochondrial oxygen consumption (non mito OCR). The analyses were performed in quadruplicate. The statistical analyses were performed using One-Way ANOVA followed by Tukey’s Multiple Comparison Test. (* = P < 0.05)

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