Skip to main content
Fig. 7 | Proteome Science

Fig. 7

From: NEK10 interactome and depletion reveal new roles in mitochondria

Fig. 7

The knock down of NEK10 changes mitochondrial reactive oxygen species (ROS) levels, and decreases citrate synthase activity. a Mitochondrial superoxide from HeLa pLKO, HeLa pLKO-sh89 and HeLa pLKO-sh90 cells was measured, using MitoSOX probe, through flow cytometry. Results represent the percentage of median fluorescence from at least three independent experiments relative to control (pLKO-empty). The statistical analyses were performed using GraphPad Prism software using Student t-test unpaired two-tailed analysis: (*) p < 0.05. b Total protein extract of HeLa pLKO, HeLa pLKO-sh89 and HeLa pLKO-sh90 cells was used to measure the citrate synthase activity with acetyl-CoA. The colorimetric assay was performed using a spectrophotometer. The graph shows analyses from n = 5 independent experiments. One-Way ANOVA followed by Tukey’s Multiple Comparison Test was used for statistical analyses (* = P < 0.05; ** = P < 0.01; *** = P < 0.001)

Back to article page