Fig. 8

Mitochondrial DNA (mtDNA) analysis of HeLa pLKO-empty, HeLa pLKO-sh89 and HeLa pLKO-sh90 cells. a The mtDNA integrity of NEK10 depleted and control cells was analyzed by Long-Extension PCR and the ratio of the amplification is shown in the graph in basal conditions. b The mtDNA integrity of cells treated with 300 μg/ml zeocin treatment for 3 h was evaluated by long-extension PCR. Results show the difference in mtDNA damage of cells with zeocin treatment from the mtDNA damage under basal conditions. The statistical analysis is a comparison to the control wild type cells. c The number of mitochondrial DNA copy number by the different cells analyzed by Real-Time quantitative polymerase chain reaction (qPCR). Both graphs show analyses from n = 3 independent experiments. Statistical analyses were made by One-Way ANOVA and the Tukey’s Multiple Comparison Test. * = P < 0.05; ** = P < 0.01