Table 2 Comparison of solvents and kits required, total time consumed and ease of handling in five protein extraction methods

Trizol method

Trizol reagent, chloroform, ethanol, isopropanol and lysis buffer

3 h

Easy to follow the procedures on manual

Lysis buffer method

Lysis buffer

0.5 h

With only a few steps

Solvent Preparation is needed prior to extraction

TCA/acetone precipitation

10% TCA in acetone, cold acetone, DTT and lysis buffer

Overnight^a

(12–20 h)

Solvent Preparation is needed prior to extraction

Aliquot Trizol method

Trizol reagent, chloroform, ethanol, isopropanol and lysis buffer

4 h

Handling multiple samples simultaneously

Trizol method with a commercial clean-up kit

Trizol reagent, chloroform, ethanol, isopropanol, 2-D clean-up kit, Mili-Q water and lysis buffer

6 h

Many steps in the clean-up procedure