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Table 2 Comparison of solvents and kits required, total time consumed and ease of handling in five protein extraction methods

From: Production of high-quality two-dimensional gel electrophoresis profile for marine medaka samples by using Trizol-based protein extraction approaches

Protein Extraction MethodSolvents and Kits RequiredTotal Time ConsumedEase of Handling
Trizol methodTrizol reagent, chloroform, ethanol, isopropanol and lysis buffer3 hEasy to follow the procedures on manual
Lysis buffer methodLysis buffer0.5 hWith only a few steps
Solvent Preparation is needed prior to extraction
TCA/acetone precipitation10% TCA in acetone, cold acetone, DTT and lysis bufferOvernighta
(12–20 h)
Solvent Preparation is needed prior to extraction
Aliquot Trizol methodTrizol reagent, chloroform, ethanol, isopropanol and lysis buffer4 hHandling multiple samples simultaneously
Trizol method with a commercial clean-up kitTrizol reagent, chloroform, ethanol, isopropanol, 2-D clean-up kit, Mili-Q water and lysis buffer6 hMany steps in the clean-up procedure
  1. aTCA/acetone precipitation used in this study was an overnight method. A normal TCA/acetone precipitation protocol could be completed within a few hours