Protein Extraction Method | No. of Protein Spots | Quality of Background | Resolution of Proteins |
---|---|---|---|
Trizol method | 1447 (32.47) | + | + |
Lysis buffer method | 1629 (112.59) | – | + |
TCA/acetone precipitation | 1154 (96.91)* | – | – |
Aliquot Trizol method | 1550 (65.91) | ++ | ++ |
Trizol method with a commercial clean-up kit | 1628 (40.28) | +++ | +++ |