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Table 1 Predominant proteins identified by MS/MS in 2-DE protein spots from the KCl-soluble fraction of Butte 86 flour. All proteins had identification scores greater than 99% probability with at least 4 peptides as determined by Scaffold. Positions of spots in 2-DE are shown in Fig. 2. Identities of all proteins found in each spot are reported in Additional file 1

From: Deciphering the immunogenic potential of wheat flour: a reference map of the salt-soluble proteome from the U.S. wheat Butte 86

Spot Number Predominant Protein Accession #a Source # Unique peptides # Spectra % Coverage
1 pyruvate phosphate dikinase XP_0201883631 Ae. tauschii 19 50 24
2 pyruvate phosphate dikinase XP_0201883631 Ae. tauschii 19 83 21
3 pyruvate phosphate dikinase XP_0201883631 Ae. tauschii 20 101 26
4 aconitate hydratase XP_020176302 Ae. tauschii 13 31 15
5 no ID      
6 heat shock protein 101 AAD22629 T.aestivum 26 67 31
7 elongation factor 2 EMS59408 T. urartu 20 52 25
8 elongation factor 2 EMS59408 T. urartu 11 25 13
9 embryonic protein DC-8-like XP_020196294 Ae. tauschii 14 50 24
10 embryonic protein DC-8-like XP_020196294 Ae. tauschii 20 59 29
11 embryonic protein DC-8-like XP_020196294 Ae. tauschii 17 65 31
12b embryonic protein DC-8-like XP_020196294 Ae. tauschii 30 85 38
13 embryonic protein DC-8-like XP_020196294 Ae. tauschii 42 131 50
14 heat shock protein 90 ABG57075 T.aestivum 19 64 26
15 heat shock protein 70 XP_020151532 Ae. tauschii 43 209 48
16 heat shock protein 70 EMS51416 T. urartu 26 85 35
17 no ID      
18 beta-D-glucan exohydrolase AAM13694 T.aestivum 25 84 34
19 beta-D-glucan exohydrolase AAM13694 T.aestivum 24 60 33
20 beta-D-xylosidase XP_020155854 Ae. tauschii 17 44 23
21c glucose and ribitol dehydrogenase pir T06212 Hordeum vulgare 7 19 28
22 no ID      
23 glucose-6-P isomerase ABE41790 T.aestivum 22 67 37
24 glucose-6-P isomerase ABE41790 T.aestivum 21 59 38
25 globulin 3A AFM30909 T.aestivum 12 38 24
26b,c beta-amylase XP_020197275 Ae. tauschii 64 397 70
27c,d beta-amylase XP_020197275 Ae. tauschii 46 287 58
28 beta-amylase EMS68884 T. urartu 21 83 37
29 beta-amylase EMS68884 T. urartu 22 88 40
30 ketol-acid reductoisomerase XP_020174215 Ae. tauschii 38 111 47
31 leucine aminopeptidase 2 EMS53149 T. urartu 26 61 47
32 atp1 ACA62607 T.aestivum 20 67 38
33 aldehyde dehydrogenase AKE36953 T.aestivum 6 17 16
34 omega gliadin AKB95614 T. urartu 18 34 51
35 enolase XP_020163593 Ae. tauschii 33 135 78
36 enolase XP_020163593 Ae. tauschii 55 164 89
37 enolase BAJ85279 Hordeum vulgare 45 167 83
38c enolase BAJ85279 Hordeum vulgare 19 62 63
39 alanine aminotransferase XP_020147857 Ae. tauschii 38 104 66
40 leghemoglobin reductase XP_020185250 Ae. tauschii 35 139 69
41 cupincin XP_020147625 Ae. tauschii 16 42 33
42 cupincin XP_020147625 Ae. tauschii 30 90 47
43 cupincin XP_020147625 Ae. tauschii 35 105 51
44 cupincin XP_020147625 Ae. tauschii 33 151 47
45d cupincin XP_020147625 Ae. tauschii 35 192 53
46 cupincin XP_020147625 Ae. tauschii 38 112 53
47 globulin-3A AFM30909 T.aestivum 34 136 45
48 globulin-3A AFM30909 T.aestivum 35 107 38
49 globulin-3A AFM30909 T.aestivum 43 147 46
50 globulin-3A AFM30909 T.aestivum 25 77 28
51 translation elongation factor 1 alpha AAA34306 T.aestivum 25 109 44
52 globulin 3 ACJ65514 T.aestivum 5 13 8
53 globulin-3A AFM30909 T.aestivum 25 83 28
54d,e globulin-3A AFM30909 T.aestivum 23 107 35
55d,f LMW-GS ACA63869 T.aestivum 27 84 53
56 globulin-3A AFM30909 T.aestivum 7 23 14
57 LMW-GS ABC84366 T.aestivum 28 54 60
58 LMW-GS ABC84366 T.aestivum 9 14 29
59 ERBB-3 binding protein XP_020191236 Ae. tauschii 11 53 29
60d gamma gliadin BU-gamma-5 T.aestivum 15 38 41
61d gamma gliadin BU-gamma5 T.aestivum 15 52 41
62 triticin ACB41345 T.aestivum 15 49 26
63 isocitrate dehydrogenase AMP82030 T.aestivum 11 41 27
64 serpin EMS54555 T. uratu 34 102 68
65 serpin CAB52709 T.aestivum 51 213 78
66 serpin ACN59485 T.aestivum 35 109 66
67 serpin ACN59485 T.aestivum 90 358 85
68 serpin ACN59485 T.aestivum 55 162 78
69 serpin ACN59484 T.aestivum 16 50 40
70 serpin ACN59484 T.aestivum 25 75 48
71 serpin AFC89429 T.aestivum 30 67 63
72 serpin AFC89429 T.aestivum 87 268 86
73 serpin AFC89429 T.aestivum 65 207 82
74 serpin AFC89429 T.aestivum 11 36 32
75 glycosyltransferase 75 ADK56176 T.aestivum 7 18 22
76 serpin CAA90071 T.aestivum 31 123 61
77 serpin CAA90071 T.aestivum 31 61 61
78 serpin EMS46390 T. uratu 39 115 67
79 alpha gliadin BAM08450 T.aestivum 10 23 34
80b,d,f alpha gliadin CAY54134 T.aestivum 25 104 45
81b ER molecular chaperone AGN94840 T.aestivum 30 149 34
82d,f gamma gliadin BU-gamma-1 T.aestivum 29 164 50
83d,f,g alpha gliadin BU-alpha-23 T.aestivum 38 107 63
84d LMW-GS ABC84361 T.aestivum 17 48 33
85 no ID      
86f LMW-GS ACJ76984 T. dicoccoides 7 25 16
87d,f gamma gliadin AEA30015 T.aestivum 24 78 53
88 alpha gliadin AAA96522 T.aestivum 11 24 42
89 peroxidase XP_020147267 Ae. tauschii 38 173 53
90 peroxidase AAM88383 T.aestivum 47 125 57
91b,d,e peroxidase AAM88383 T.aestivum 62 197 60
92 aldose 1 epimerase XP_020180146 Ae. tauschii 9 24 22
93 globulin 3A AFM30909 T.aestivum 19 57 25
94 63 kd globulin XP-020163670 Ae. tauschii 24 88 27
95 farinin XP_020147251 (BU-farinin-1) Ae. tauschii 9 49 31
96 63 kd globulin XP-020163670 Ae. tauschii 24 106 28
97 aspartic proteinase XP_020157026 Ae. tauschii 9 27 19
98g aspartic proteinase XP_020157026 Ae. tauschii 5 13 13
99 cupincin XP_020147625 Ae. tauschii 8 31 18
100 peroxidase AAM88383 T.aestivum 13 36 34
101b,d,h glyceraldehyde 3-P dehydrogenase ALE18234 T.aestivum 49 164 65
102b,d glyceraldehyde 3-P dehydrogenase ANW11922 T.aestivum 79 230 92
103b,d glyceraldehyde 3-P dehydrogenase ALE18234 T.aestivum 37 85 68
104b,d fructose bisphosphate aldolase CDM85265 T.aestivum 60 197 73
105b,d glyceraldehyde 3-P dehydrogenase ABQ81648 T.aestivum 56 208 73
106b,d,f alpha gliadin CS-alpha-A1 T.aestivum 70 215 77
107 malate dehydrogenase XP_020196362 Ae. tauschii 6 17 17
108 guanine nucleotide binding protein CDM83867 T.aestivum 11 23 40
109 malate dehydrogenase XP_020157252 Ae. tauschii 44 149 73
110 malate dehydrogenase AAT64932 T.aestivum 21 53 54
111 desiccation-related protein XP_020169835 Ae. tauschii 16 37 35
112 desiccation-related protein XP_020169835 Ae. tauschii 11 34 31
113 lactoylglutathione lyase XP_020185375 Ae. tauschii 13 38 41
114 lactoylglutathione lyase XP_020185375 Ae. tauschii 51 149 79
115 lactoylglutathione lyase XP_020185375 Ae. tauschii 16 54 44
116 lactoylglutathione lyase EMS56223 T. uratu 7 19 21
117 glucose and ribitol dehydrogenase XP_020188730 Ae. tauschii 16 41 46
118 glucose and ribitol dehydrogenase XP_020188730 Ae. tauschii 28 107 61
119 glucose and ribitol dehydrogenase XP_020188730 Ae. tauschii 31 127 67
120 glucose and ribitol dehydrogenase XP_020188730 Ae. tauschii 29 101 65
121 proteosome subunit alpha type 1 BAJ85935 Hordeum vulgare 10 28 39
122 glucose and ribitol dehydrogenase BAJ85825 Hordeum vulgare 15 64 39
123 dehydrin AOM63239 T.aestivum 12 29 61
124 purinin ADA62372 (BU-purinin-3) T.aestivum 14 105 40
125 19 kd globulin XP_020162707 Ae. tauschii 12 65 61
126 oil body associated protein XP_020196637 Ae. tauschii 24 74 81
127 19 kd globulin XP_020162707 Ae. tauschii 9 62 50
128 xylanase inhibitor AGN71004 T.aestivum 34 127 64
129 globulin-1 ABG68034 T.aestivum 12 55 46
130 chitinase XP_020181917 Ae. tauschii 19 108 41
131 globulin AAR95703 T. turgidum 11 65 50
132 globulin AAR95703 T. turgidum 10 72 50
133 tritin BAA02948 T.aestivum 53 316 75
134 farinin XP_020147251 (BU-farinin-1) Ae. tauschii 7 35 21
135 secretory protein XP_020161391 Ae. tauschii 9 23 38
136 secretory protein XP_020161391 Ae. tauschii 5 14 29
137 cyclophilin A AAK49426 T.aestivum 14 84 57
138 chitinase XP_020181917 Ae. tauschii 15 28 32
139 chitinase AAX83263 T.aestivum 20 73 48
140 AAI prf 1207200A (WASI BU-1) T.aestivum 39 234 82
141 AAI prf 1207200A (WASI BU-1) T.aestivum 14 72 51
142 AAI prf 1207200A (WASI BU-1) T.aestivum 31 112 71
143 peroxiredoxin ACE82290 T.aestivum 21 91 67
144 1-cys peroxiredoxin AAQ74769 T.aestivum 21 73 65
145 purinin EMS62569 (BU-purinin-1) T. urartu 22 98 68
146 purinin EMS62569 (BU-purinin-1) T. urartu 9 37 52
147 triosephosphate isomerase CDM81250 T.aestivum 31 102 70
148 oil body associated protein XP_020147075 Ae. tauschii 30 103 84
149 late embryogenesis abundant protein XP_020193122 Ae. tauschii 10 22 55
150 late embryogenesis abundant protein XP_020193122 Ae. tauschii 14 39 62
151 late embryogenesis abundant protein BAK04960 Hordeum vulgare 6 16 28
152 glutathionine-S-transferase XP_020196644 Ae. tauschii 15 57 49
153 glutathionine-S-transferase XP_020196644 Ae. tauschii 51 214 60
154 glutathionine-S-transferase XP_020196644 Ae. tauschii 21 65 53
155 lysosomal thiol reductase XP_020155412 Ae. tauschii 15 73 52
156 nucleoside diphosphate kinase EMS63724 T. urartu 5 16 41
157 AAI AAR10959 (WTAI-CM3 BU-1) T.aestivum 37 388 74
158 no ID      
159 AAI CAI84642 (WDAI BU-1) T.aestivum 8 24 66
160 no ID      
161 no ID      
162 no ID      
163 ubiquitin AAP50253 T.aestivum 10 56 83
164 AAI AAV39514 (WDAI BU-1) T.aestivum 26 244 100
165 AAI AAV39517 (WDAI BU-1) T.aestivum 22 101 88
166 AAI sp P16851 (WTAI-CM2 BU-1) T.aestivum 24 107 74
167 superoxide dismutase AFF27607 T.aestivum 18 61 66
168 globulin-1 EMS62417 T. urartu 12 53 15
169 AAI ABO45967 (WMAI BU-1) T.aestivum 25 135 99
170 AAI ABI54484 (WDAI BU-3) T. dicoccoides 14 58 96
171 AAI ABO45967 (WMAI BU-1) T.aestivum 19 116 98
172 AAI AAV91972 (WDAI BU-4) T.aestivum 37 198 100
173 AAI ACM41418 (WTAI-CM16 BU-1) T. macha 34 228 80
174 AAI CAA42453 (WTAI-CM-17 BU-1) T.aestivum 25 133 77
175 AAI CAA42453 (WTAI-CM-17 BU-1) T.aestivum 13 59 50
176 AAI ABO45967 (WMAI BU-1) T.aestivum 4 17 36
177 AAI sp P16851 (WTAI-CM2 BU-1) T.aestivum 7 25 44
178 homocysteine methyltransferase EMS51950 T. urartu 20 76 27
189d,f alpha gliadin BU-alpha-3 T.aestivum 15 44 35
190d,e alpha gliadin BAM08458 T.aestivum 23 73 50
191b,d alpha gliadin BU-alpha-4 T.aestivum 19 53 46
  1. a Accession numbers beginning with BU are from Butte 86 and CS are from Chinese Spring. All other accession numbers are from NCBI. The Butte 86 accession is shown in parentheses in cases where a sequence from NCBI is identical to a sequence from Butte 86. Gliadin, purinin and farinin sequences from Butte 86 are from [19]. AAI sequences from Butte 86 are from [22]
  2. b a gluten protein with less than half the number of unique peptides found in the predominant protein was also identified in spot
  3. c also contains an omega gliadin
  4. d replicate sample was digested with thermolysin and analyzed by MS/MS
  5. e also contains a LMW-GS
  6. f also contains a non-gluten protein
  7. g also contains a gamma gliadin
  8. h also contains an alpha gliadin