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Table 1 Predominant proteins identified by MS/MS in 2-DE protein spots from the KCl-soluble fraction of Butte 86 flour. All proteins had identification scores greater than 99% probability with at least 4 peptides as determined by Scaffold. Positions of spots in 2-DE are shown in Fig. 2. Identities of all proteins found in each spot are reported in Additional file 1

From: Deciphering the immunogenic potential of wheat flour: a reference map of the salt-soluble proteome from the U.S. wheat Butte 86

Spot Number

Predominant Protein

Accession #a

Source

# Unique peptides

# Spectra

% Coverage

1

pyruvate phosphate dikinase

XP_0201883631

Ae. tauschii

19

50

24

2

pyruvate phosphate dikinase

XP_0201883631

Ae. tauschii

19

83

21

3

pyruvate phosphate dikinase

XP_0201883631

Ae. tauschii

20

101

26

4

aconitate hydratase

XP_020176302

Ae. tauschii

13

31

15

5

no ID

     

6

heat shock protein 101

AAD22629

T.aestivum

26

67

31

7

elongation factor 2

EMS59408

T. urartu

20

52

25

8

elongation factor 2

EMS59408

T. urartu

11

25

13

9

embryonic protein DC-8-like

XP_020196294

Ae. tauschii

14

50

24

10

embryonic protein DC-8-like

XP_020196294

Ae. tauschii

20

59

29

11

embryonic protein DC-8-like

XP_020196294

Ae. tauschii

17

65

31

12b

embryonic protein DC-8-like

XP_020196294

Ae. tauschii

30

85

38

13

embryonic protein DC-8-like

XP_020196294

Ae. tauschii

42

131

50

14

heat shock protein 90

ABG57075

T.aestivum

19

64

26

15

heat shock protein 70

XP_020151532

Ae. tauschii

43

209

48

16

heat shock protein 70

EMS51416

T. urartu

26

85

35

17

no ID

     

18

beta-D-glucan exohydrolase

AAM13694

T.aestivum

25

84

34

19

beta-D-glucan exohydrolase

AAM13694

T.aestivum

24

60

33

20

beta-D-xylosidase

XP_020155854

Ae. tauschii

17

44

23

21c

glucose and ribitol dehydrogenase

pir T06212

Hordeum vulgare

7

19

28

22

no ID

     

23

glucose-6-P isomerase

ABE41790

T.aestivum

22

67

37

24

glucose-6-P isomerase

ABE41790

T.aestivum

21

59

38

25

globulin 3A

AFM30909

T.aestivum

12

38

24

26b,c

beta-amylase

XP_020197275

Ae. tauschii

64

397

70

27c,d

beta-amylase

XP_020197275

Ae. tauschii

46

287

58

28

beta-amylase

EMS68884

T. urartu

21

83

37

29

beta-amylase

EMS68884

T. urartu

22

88

40

30

ketol-acid reductoisomerase

XP_020174215

Ae. tauschii

38

111

47

31

leucine aminopeptidase 2

EMS53149

T. urartu

26

61

47

32

atp1

ACA62607

T.aestivum

20

67

38

33

aldehyde dehydrogenase

AKE36953

T.aestivum

6

17

16

34

omega gliadin

AKB95614

T. urartu

18

34

51

35

enolase

XP_020163593

Ae. tauschii

33

135

78

36

enolase

XP_020163593

Ae. tauschii

55

164

89

37

enolase

BAJ85279

Hordeum vulgare

45

167

83

38c

enolase

BAJ85279

Hordeum vulgare

19

62

63

39

alanine aminotransferase

XP_020147857

Ae. tauschii

38

104

66

40

leghemoglobin reductase

XP_020185250

Ae. tauschii

35

139

69

41

cupincin

XP_020147625

Ae. tauschii

16

42

33

42

cupincin

XP_020147625

Ae. tauschii

30

90

47

43

cupincin

XP_020147625

Ae. tauschii

35

105

51

44

cupincin

XP_020147625

Ae. tauschii

33

151

47

45d

cupincin

XP_020147625

Ae. tauschii

35

192

53

46

cupincin

XP_020147625

Ae. tauschii

38

112

53

47

globulin-3A

AFM30909

T.aestivum

34

136

45

48

globulin-3A

AFM30909

T.aestivum

35

107

38

49

globulin-3A

AFM30909

T.aestivum

43

147

46

50

globulin-3A

AFM30909

T.aestivum

25

77

28

51

translation elongation factor 1 alpha

AAA34306

T.aestivum

25

109

44

52

globulin 3

ACJ65514

T.aestivum

5

13

8

53

globulin-3A

AFM30909

T.aestivum

25

83

28

54d,e

globulin-3A

AFM30909

T.aestivum

23

107

35

55d,f

LMW-GS

ACA63869

T.aestivum

27

84

53

56

globulin-3A

AFM30909

T.aestivum

7

23

14

57

LMW-GS

ABC84366

T.aestivum

28

54

60

58

LMW-GS

ABC84366

T.aestivum

9

14

29

59

ERBB-3 binding protein

XP_020191236

Ae. tauschii

11

53

29

60d

gamma gliadin

BU-gamma-5

T.aestivum

15

38

41

61d

gamma gliadin

BU-gamma5

T.aestivum

15

52

41

62

triticin

ACB41345

T.aestivum

15

49

26

63

isocitrate dehydrogenase

AMP82030

T.aestivum

11

41

27

64

serpin

EMS54555

T. uratu

34

102

68

65

serpin

CAB52709

T.aestivum

51

213

78

66

serpin

ACN59485

T.aestivum

35

109

66

67

serpin

ACN59485

T.aestivum

90

358

85

68

serpin

ACN59485

T.aestivum

55

162

78

69

serpin

ACN59484

T.aestivum

16

50

40

70

serpin

ACN59484

T.aestivum

25

75

48

71

serpin

AFC89429

T.aestivum

30

67

63

72

serpin

AFC89429

T.aestivum

87

268

86

73

serpin

AFC89429

T.aestivum

65

207

82

74

serpin

AFC89429

T.aestivum

11

36

32

75

glycosyltransferase 75

ADK56176

T.aestivum

7

18

22

76

serpin

CAA90071

T.aestivum

31

123

61

77

serpin

CAA90071

T.aestivum

31

61

61

78

serpin

EMS46390

T. uratu

39

115

67

79

alpha gliadin

BAM08450

T.aestivum

10

23

34

80b,d,f

alpha gliadin

CAY54134

T.aestivum

25

104

45

81b

ER molecular chaperone

AGN94840

T.aestivum

30

149

34

82d,f

gamma gliadin

BU-gamma-1

T.aestivum

29

164

50

83d,f,g

alpha gliadin

BU-alpha-23

T.aestivum

38

107

63

84d

LMW-GS

ABC84361

T.aestivum

17

48

33

85

no ID

     

86f

LMW-GS

ACJ76984

T. dicoccoides

7

25

16

87d,f

gamma gliadin

AEA30015

T.aestivum

24

78

53

88

alpha gliadin

AAA96522

T.aestivum

11

24

42

89

peroxidase

XP_020147267

Ae. tauschii

38

173

53

90

peroxidase

AAM88383

T.aestivum

47

125

57

91b,d,e

peroxidase

AAM88383

T.aestivum

62

197

60

92

aldose 1 epimerase

XP_020180146

Ae. tauschii

9

24

22

93

globulin 3A

AFM30909

T.aestivum

19

57

25

94

63 kd globulin

XP-020163670

Ae. tauschii

24

88

27

95

farinin

XP_020147251 (BU-farinin-1)

Ae. tauschii

9

49

31

96

63 kd globulin

XP-020163670

Ae. tauschii

24

106

28

97

aspartic proteinase

XP_020157026

Ae. tauschii

9

27

19

98g

aspartic proteinase

XP_020157026

Ae. tauschii

5

13

13

99

cupincin

XP_020147625

Ae. tauschii

8

31

18

100

peroxidase

AAM88383

T.aestivum

13

36

34

101b,d,h

glyceraldehyde 3-P dehydrogenase

ALE18234

T.aestivum

49

164

65

102b,d

glyceraldehyde 3-P dehydrogenase

ANW11922

T.aestivum

79

230

92

103b,d

glyceraldehyde 3-P dehydrogenase

ALE18234

T.aestivum

37

85

68

104b,d

fructose bisphosphate aldolase

CDM85265

T.aestivum

60

197

73

105b,d

glyceraldehyde 3-P dehydrogenase

ABQ81648

T.aestivum

56

208

73

106b,d,f

alpha gliadin

CS-alpha-A1

T.aestivum

70

215

77

107

malate dehydrogenase

XP_020196362

Ae. tauschii

6

17

17

108

guanine nucleotide binding protein

CDM83867

T.aestivum

11

23

40

109

malate dehydrogenase

XP_020157252

Ae. tauschii

44

149

73

110

malate dehydrogenase

AAT64932

T.aestivum

21

53

54

111

desiccation-related protein

XP_020169835

Ae. tauschii

16

37

35

112

desiccation-related protein

XP_020169835

Ae. tauschii

11

34

31

113

lactoylglutathione lyase

XP_020185375

Ae. tauschii

13

38

41

114

lactoylglutathione lyase

XP_020185375

Ae. tauschii

51

149

79

115

lactoylglutathione lyase

XP_020185375

Ae. tauschii

16

54

44

116

lactoylglutathione lyase

EMS56223

T. uratu

7

19

21

117

glucose and ribitol dehydrogenase

XP_020188730

Ae. tauschii

16

41

46

118

glucose and ribitol dehydrogenase

XP_020188730

Ae. tauschii

28

107

61

119

glucose and ribitol dehydrogenase

XP_020188730

Ae. tauschii

31

127

67

120

glucose and ribitol dehydrogenase

XP_020188730

Ae. tauschii

29

101

65

121

proteosome subunit alpha type 1

BAJ85935

Hordeum vulgare

10

28

39

122

glucose and ribitol dehydrogenase

BAJ85825

Hordeum vulgare

15

64

39

123

dehydrin

AOM63239

T.aestivum

12

29

61

124

purinin

ADA62372 (BU-purinin-3)

T.aestivum

14

105

40

125

19 kd globulin

XP_020162707

Ae. tauschii

12

65

61

126

oil body associated protein

XP_020196637

Ae. tauschii

24

74

81

127

19 kd globulin

XP_020162707

Ae. tauschii

9

62

50

128

xylanase inhibitor

AGN71004

T.aestivum

34

127

64

129

globulin-1

ABG68034

T.aestivum

12

55

46

130

chitinase

XP_020181917

Ae. tauschii

19

108

41

131

globulin

AAR95703

T. turgidum

11

65

50

132

globulin

AAR95703

T. turgidum

10

72

50

133

tritin

BAA02948

T.aestivum

53

316

75

134

farinin

XP_020147251 (BU-farinin-1)

Ae. tauschii

7

35

21

135

secretory protein

XP_020161391

Ae. tauschii

9

23

38

136

secretory protein

XP_020161391

Ae. tauschii

5

14

29

137

cyclophilin A

AAK49426

T.aestivum

14

84

57

138

chitinase

XP_020181917

Ae. tauschii

15

28

32

139

chitinase

AAX83263

T.aestivum

20

73

48

140

AAI

prf 1207200A (WASI BU-1)

T.aestivum

39

234

82

141

AAI

prf 1207200A (WASI BU-1)

T.aestivum

14

72

51

142

AAI

prf 1207200A (WASI BU-1)

T.aestivum

31

112

71

143

peroxiredoxin

ACE82290

T.aestivum

21

91

67

144

1-cys peroxiredoxin

AAQ74769

T.aestivum

21

73

65

145

purinin

EMS62569 (BU-purinin-1)

T. urartu

22

98

68

146

purinin

EMS62569 (BU-purinin-1)

T. urartu

9

37

52

147

triosephosphate isomerase

CDM81250

T.aestivum

31

102

70

148

oil body associated protein

XP_020147075

Ae. tauschii

30

103

84

149

late embryogenesis abundant protein

XP_020193122

Ae. tauschii

10

22

55

150

late embryogenesis abundant protein

XP_020193122

Ae. tauschii

14

39

62

151

late embryogenesis abundant protein

BAK04960

Hordeum vulgare

6

16

28

152

glutathionine-S-transferase

XP_020196644

Ae. tauschii

15

57

49

153

glutathionine-S-transferase

XP_020196644

Ae. tauschii

51

214

60

154

glutathionine-S-transferase

XP_020196644

Ae. tauschii

21

65

53

155

lysosomal thiol reductase

XP_020155412

Ae. tauschii

15

73

52

156

nucleoside diphosphate kinase

EMS63724

T. urartu

5

16

41

157

AAI

AAR10959 (WTAI-CM3 BU-1)

T.aestivum

37

388

74

158

no ID

     

159

AAI

CAI84642 (WDAI BU-1)

T.aestivum

8

24

66

160

no ID

     

161

no ID

     

162

no ID

     

163

ubiquitin

AAP50253

T.aestivum

10

56

83

164

AAI

AAV39514 (WDAI BU-1)

T.aestivum

26

244

100

165

AAI

AAV39517 (WDAI BU-1)

T.aestivum

22

101

88

166

AAI

sp P16851 (WTAI-CM2 BU-1)

T.aestivum

24

107

74

167

superoxide dismutase

AFF27607

T.aestivum

18

61

66

168

globulin-1

EMS62417

T. urartu

12

53

15

169

AAI

ABO45967 (WMAI BU-1)

T.aestivum

25

135

99

170

AAI

ABI54484 (WDAI BU-3)

T. dicoccoides

14

58

96

171

AAI

ABO45967 (WMAI BU-1)

T.aestivum

19

116

98

172

AAI

AAV91972 (WDAI BU-4)

T.aestivum

37

198

100

173

AAI

ACM41418 (WTAI-CM16 BU-1)

T. macha

34

228

80

174

AAI

CAA42453 (WTAI-CM-17 BU-1)

T.aestivum

25

133

77

175

AAI

CAA42453 (WTAI-CM-17 BU-1)

T.aestivum

13

59

50

176

AAI

ABO45967 (WMAI BU-1)

T.aestivum

4

17

36

177

AAI

sp P16851 (WTAI-CM2 BU-1)

T.aestivum

7

25

44

178

homocysteine methyltransferase

EMS51950

T. urartu

20

76

27

189d,f

alpha gliadin

BU-alpha-3

T.aestivum

15

44

35

190d,e

alpha gliadin

BAM08458

T.aestivum

23

73

50

191b,d

alpha gliadin

BU-alpha-4

T.aestivum

19

53

46

  1. a Accession numbers beginning with BU are from Butte 86 and CS are from Chinese Spring. All other accession numbers are from NCBI. The Butte 86 accession is shown in parentheses in cases where a sequence from NCBI is identical to a sequence from Butte 86. Gliadin, purinin and farinin sequences from Butte 86 are from [19]. AAI sequences from Butte 86 are from [22]
  2. b a gluten protein with less than half the number of unique peptides found in the predominant protein was also identified in spot
  3. c also contains an omega gliadin
  4. d replicate sample was digested with thermolysin and analyzed by MS/MS
  5. e also contains a LMW-GS
  6. f also contains a non-gluten protein
  7. g also contains a gamma gliadin
  8. h also contains an alpha gliadin