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Fig. 1 | Proteome Science

Fig. 1

From: Label-free quantitative proteomics of Sorghum bicolor reveals the proteins strengthening plant defense against insect pest Chilo partellus

Fig. 1

Experimental overview| Three S. bicolor genotypes (ICSV700, IS2205, Swarna) with varied insect-susceptibility were planted in the field in randomized block design. Insect-infestation was carried out with the Bazooka applicator and leaves were collected 5 days post-infestation. Leaves from 5 plants were pooled and considered as a biological replicate, and two such replicates were used in the analysis. The proteins were isolated from leaves and subjected to in-solution digestion. The MS-MS analysis was performed with SYNAPT HDMSE and S. bicolor proteome was used for protein identification. Proteins were analyzed using non-parametric multivariate tests using R. Further, gene ontology and gene expression analysis of proteins were performed

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