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Table 3 Differentially abundant S. bicolor proteins identified from the S-plot analysis of the in solution proteomics data

From: Label-free quantitative proteomics of Sorghum bicolor reveals the proteins strengthening plant defense against insect pest Chilo partellus

Status

Protein Key

Protein Accession No.

Name of protein/similar protein

Function/ GO

Up

372

C5X1U2

Calmodulin

Calcium ion binding (GO:0005509), calcium-mediated signaling (GO:0019722)

Up

656

C5YSK7

similar to Pathogenesis related protein 5

Defense response (GO:0006952)

Up

1292

C5YBE9

Chitin-binding type-1 domain-containing protein

Chitinase activity (GO:0004568)

Up

1510

C5YSK6

similar to Thaumatin like pathogenesis related protein 1

Defense response (GO:0006952)

Up

5767

C5Z0N8

Peroxidase

2 phenolic donor + H2O2 = 2 phenolic radical donor + 2 H2

Up

6674

C5XHS1

similar to β-1,3-glucanase

Hydrolysis of O-glycosyl compounds, Carbohydrate metabolic process

Up

9254

C5XCE2

similar to Zeamatin-like protein

Inhibition of trypsin and α-amylases, Defense response (GO:0006952)

Up

9604

C5Z469

Peroxidase

2 phenolic donor + H2O2 = 2 phenolic radical donor + 2 H2O

Up

10,330

C5WZ07

similar to Glutathione S-transferase

Glutathione transferase activity (GO:0004364)

Up

11,895

C5YSV2

similar to Thaumatin like pathogenesis related protein 5

Defense response (GO:0006952)

Up

12,145

C5YYT5

similar to 60S acidic ribosomal protein P2B isoform X1

 

Up

13,645

C5Z3A0

SCP domain-containing protein

similar to pathogenesis-related protein

Up

14,437

C5WWX5

similar to Histone2A

 

Up

17,199

C5Z9A2

similar to Thylakoid lumenal 16.5 kDa protein

Photosystem II repair (GO:0010206)

Up

19,206

C5WT31

similar to DPP6 N-terminal domain-like protein

 

Up

23,877

C5YLY5

similar to Ribosome-recycling factor

 

Up

26,619

C5Y817

similar to Carboxyl terminal peptidase precursor

Peptidase activity

Up

26,971

C5X8S2

SCP domain-containing protein

Cysteine rich secretory protein, allergen V5/Tpx-1

Up

28,788

C5WQE1

similar to α-amylase/ trypsin inhibitor

 

Up

30,151

C5Z8N5

Expansin-like EG45 domain-containing protein

Chitinase activity

Up

31,567

C5YGE3

similar to Abscisic acid stress ripening 3

 

Up

31,569

C5Y5D6

Barwin domain-containing protein

Defense response to bacterium (GO:0042742) or fungus (GO:0050832)

Down

34

A1E9V4

Cytochrome b6

Component of the cytochrome b6-f complex

Down

102

A1E9W6

50S ribosomal protein L2, chloroplastic

Mitochondrial translation (GO:0032543)

Down

121

A1E9W0

30S ribosomal protein S8, chloroplastic

Translation (GO:0006412)

Down

260

C5YH12

Caffeic acid O-methyltransferase

Flavonol biosynthetic process (GO:0051555)

Down

353

C5XYX5

similar to 60S ribosomal protein L11–1

Translation (GO:0006412)

Down

1163

C5X1Q1

similar to Hydroxyproline-rich glycoprotein family protein

 

Down

1442

C5Y065

Lipase_3 domain-containing protein

Lipid metabolic process (GO:0006629)

Down

1979

C5YIF8

Obg-like ATPase 1

ATPase activity (GO:00016887), Negative regulation of response to salt stress (GO:1901001)& defense response to bacterium (GO:1900425)

Down

3699

C5YRK9

similar to Pentatricopeptide repeat-containing protein

RNA modification (GO:0009451)

Down

4242

C5XW30

similar to Phorphobilinogen deaminase

It catalyzes head to tail condensation of four porphobilinogen molecules releasing 4 ammonia molecules

Down

5841

C5YRL0

Non-specific lipid transfer protein

Bifunctional protease and alpha amylase inhibitor inhibitor, lipid binding (GO:0008289) lipid transfer (GO:0006869) protein

Down

6172

C5XYT6

FAD_binding_3 domain-containing protein

FAD binding (GO:0071949),Geranylgeranyl reductase activity (GO:0045550)

Down

10,362

C5YL07

Aldedh domain-containing protein

Betaine-aldehyde dehydrogenase activity (GO:0008802), Response to anoxia (GO:0071454)

Down

11,647

C5WTC9

Ribosomal_L16 domain-containing protein

Translation (GO:0006412)

Down

12,657

C5Z267

similar to 60S ribosomal protein L9

Cytoplasmic translation (GO:0002181)

Down

14,425

C5YAD0

similar to 60S ribosomal protein L6

Cytoplasmic translation (GO:0002181)

Down

15,418

C5XEA1

similar to Fructose-bisphosphate aldolase 1, chloroplastic isoform X1

 

Down

15,466

C5YHF2

similar to Rubredoxin family protein

 

Down

15,661

C5XZ84

40S ribosomal protein S8

Translation (GO:0006412)

Down

15,716

C5WZ25

Tubulin beta chain

GTPase activity (GO:0003924), microtubule cytskeletal organization (GO:0000226)

Down

16,668

C5YAI8

Pyruvate kinase

ATP + pyruvate = ADP + H+ + phosphoenolpyruvate, Glycolytic process (GO:0006096)

Down

17,564

C5YCD5

PfkB domain-containing protein

Adenosine kinase activity (GO:0004001), Purine ribonucleoside salvage (GO:0006166)

Down

18,075

C5YXW7

Guanosine nucleotide diphosphate dissociation inhibitor

Rab GTPase binding (GO:0017137), small GTPase mediated signal transduction (GO:0007264)

Down

19,332

C5X6V0

similar to Extracellular ribonuclease LE

RNA catabolic process (GO:0006401)

Down

19,346

C5YG66

Aminomethyltransferase

Aminomethyltransferase activity (GO:0004047), Glycine decarboxylation via glycine cleavage system (GO:0019464)

Down

21,133

C5YG29

similar to 60S ribosomal protein

Translation (GO:0006412)

Down

22,396

C5YCD6

Phenylalanine ammonia-lyase

L-phenylalanine = NH4+ + trans-cinnamate, Cinnamic acid biosynthetic process (GO:0009800), L-phenylalanine catabolic process (GO:0006559)

Down

22,977

C5WT26

40S ribosomal protein S4

Translation (GO:0006412)

Down

23,733

C5YX57

40S ribosomal protein S4

Translation (GO:0006412)

Down

23,995

C5YU66

similar to Heat shock 70 kDa protein 4

Stress response

Down

24,630

C5YJP1

HATPase_c domain-containing protein

Unfolded protein binding (GO:0051082), Response to chlorate (GO:0010157), heat (GO:0009408), salt stress (GO:0009651), water deprivation (GO:0009414)

Down

25,743

C5X255

similar to Formate tetrahydrofolate ligase

 

Down

25,986

C5WXD2

similar to Protein TIC110, chloroplastic

 

Down

26,465

C5XXT8

Phenylalanine ammonia-lyase

L-phenylalanine = NH4+ + trans-cinnamate, Cinnamic acid biosynthetic process (GO:0009800), L-phenylalanine catabolic process (GO:0006559)

Down

28,031

C5XIT6

Pectinesterase

[(1 → 4)-α-D-galacturonosyl methyl ester](n) + n H2O = [(1 → 4)-α-D-galacturonosyl](n) + n H+ + n methanol, cell wall modification (GO:0042545)

Down

28,874

C5YMU8

similar to Puromycin-sensitive aminopeptidase

 

Down

29,216

C5YPW0

similar to ATP-citrate synthase

ATP binding (GO:0005524)

Down

30,618

C5WZ87

similar to Ribosomal protein S9

Translation (GO:0006412)

Down

30,990

C5XI18

S-adenosylmethionine synthase

ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine, S-adenosylmethionine biosynthetic process (GO:0006556)

Down

31,330

C5YNT6

S4 RNA-binding domain-containing protein

Translation (GO:0006412), Positive regulation of translational fidelity (GO:0045903)

Down

31,631

C5WXA8

NADPH-protochlorophyllide oxidoreductase

chlorophyllide a + NADP+ = H+ + NADPH + protochlorophyllide a

Down

31,939

C5WZQ4

similar to 50S ribosomal protein L6

Translation (GO:0006412)

Down

32,283

C5XE18

40S ribosomal protein SA

Cytoplasmic translation (GO:0002181), Translation (GO:0006412)

Down

32,318

C5YFQ2

Ribosomal_S17_N domain-containing protein

Translation (GO:0006412)

Down

32,520

C5X0S2

Uroporphyrinogen decarboxylase

4 H+ + uroporphyrinogen III = 4 CO2 + coproporphyrinogen III, Protoporphyrinogen IX biosynthetic process (GO:0006782)

Down

32,758

C5YS19

SAM_MPBQ_MSBQ_MT domain-containing protein

Methyltransferase activity (GO:0008168)

  1. The OPLS-DA analysis followed by S-plot analysis was carried out to identify proteins from S. bicolor genotypes that showed significant differential abundance. The commonly expressed proteins identified from S. bicolor genotypes in all the treatments namely steady-state and C. partellus induced were considered for this analysis