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Table 3 Differentially abundant S. bicolor proteins identified from the S-plot analysis of the in solution proteomics data

From: Label-free quantitative proteomics of Sorghum bicolor reveals the proteins strengthening plant defense against insect pest Chilo partellus

Status Protein Key Protein Accession No. Name of protein/similar protein Function/ GO
Up 372 C5X1U2 Calmodulin Calcium ion binding (GO:0005509), calcium-mediated signaling (GO:0019722)
Up 656 C5YSK7 similar to Pathogenesis related protein 5 Defense response (GO:0006952)
Up 1292 C5YBE9 Chitin-binding type-1 domain-containing protein Chitinase activity (GO:0004568)
Up 1510 C5YSK6 similar to Thaumatin like pathogenesis related protein 1 Defense response (GO:0006952)
Up 5767 C5Z0N8 Peroxidase 2 phenolic donor + H2O2 = 2 phenolic radical donor + 2 H2
Up 6674 C5XHS1 similar to β-1,3-glucanase Hydrolysis of O-glycosyl compounds, Carbohydrate metabolic process
Up 9254 C5XCE2 similar to Zeamatin-like protein Inhibition of trypsin and α-amylases, Defense response (GO:0006952)
Up 9604 C5Z469 Peroxidase 2 phenolic donor + H2O2 = 2 phenolic radical donor + 2 H2O
Up 10,330 C5WZ07 similar to Glutathione S-transferase Glutathione transferase activity (GO:0004364)
Up 11,895 C5YSV2 similar to Thaumatin like pathogenesis related protein 5 Defense response (GO:0006952)
Up 12,145 C5YYT5 similar to 60S acidic ribosomal protein P2B isoform X1  
Up 13,645 C5Z3A0 SCP domain-containing protein similar to pathogenesis-related protein
Up 14,437 C5WWX5 similar to Histone2A  
Up 17,199 C5Z9A2 similar to Thylakoid lumenal 16.5 kDa protein Photosystem II repair (GO:0010206)
Up 19,206 C5WT31 similar to DPP6 N-terminal domain-like protein  
Up 23,877 C5YLY5 similar to Ribosome-recycling factor  
Up 26,619 C5Y817 similar to Carboxyl terminal peptidase precursor Peptidase activity
Up 26,971 C5X8S2 SCP domain-containing protein Cysteine rich secretory protein, allergen V5/Tpx-1
Up 28,788 C5WQE1 similar to α-amylase/ trypsin inhibitor  
Up 30,151 C5Z8N5 Expansin-like EG45 domain-containing protein Chitinase activity
Up 31,567 C5YGE3 similar to Abscisic acid stress ripening 3  
Up 31,569 C5Y5D6 Barwin domain-containing protein Defense response to bacterium (GO:0042742) or fungus (GO:0050832)
Down 34 A1E9V4 Cytochrome b6 Component of the cytochrome b6-f complex
Down 102 A1E9W6 50S ribosomal protein L2, chloroplastic Mitochondrial translation (GO:0032543)
Down 121 A1E9W0 30S ribosomal protein S8, chloroplastic Translation (GO:0006412)
Down 260 C5YH12 Caffeic acid O-methyltransferase Flavonol biosynthetic process (GO:0051555)
Down 353 C5XYX5 similar to 60S ribosomal protein L11–1 Translation (GO:0006412)
Down 1163 C5X1Q1 similar to Hydroxyproline-rich glycoprotein family protein  
Down 1442 C5Y065 Lipase_3 domain-containing protein Lipid metabolic process (GO:0006629)
Down 1979 C5YIF8 Obg-like ATPase 1 ATPase activity (GO:00016887), Negative regulation of response to salt stress (GO:1901001)& defense response to bacterium (GO:1900425)
Down 3699 C5YRK9 similar to Pentatricopeptide repeat-containing protein RNA modification (GO:0009451)
Down 4242 C5XW30 similar to Phorphobilinogen deaminase It catalyzes head to tail condensation of four porphobilinogen molecules releasing 4 ammonia molecules
Down 5841 C5YRL0 Non-specific lipid transfer protein Bifunctional protease and alpha amylase inhibitor inhibitor, lipid binding (GO:0008289) lipid transfer (GO:0006869) protein
Down 6172 C5XYT6 FAD_binding_3 domain-containing protein FAD binding (GO:0071949),Geranylgeranyl reductase activity (GO:0045550)
Down 10,362 C5YL07 Aldedh domain-containing protein Betaine-aldehyde dehydrogenase activity (GO:0008802), Response to anoxia (GO:0071454)
Down 11,647 C5WTC9 Ribosomal_L16 domain-containing protein Translation (GO:0006412)
Down 12,657 C5Z267 similar to 60S ribosomal protein L9 Cytoplasmic translation (GO:0002181)
Down 14,425 C5YAD0 similar to 60S ribosomal protein L6 Cytoplasmic translation (GO:0002181)
Down 15,418 C5XEA1 similar to Fructose-bisphosphate aldolase 1, chloroplastic isoform X1  
Down 15,466 C5YHF2 similar to Rubredoxin family protein  
Down 15,661 C5XZ84 40S ribosomal protein S8 Translation (GO:0006412)
Down 15,716 C5WZ25 Tubulin beta chain GTPase activity (GO:0003924), microtubule cytskeletal organization (GO:0000226)
Down 16,668 C5YAI8 Pyruvate kinase ATP + pyruvate = ADP + H+ + phosphoenolpyruvate, Glycolytic process (GO:0006096)
Down 17,564 C5YCD5 PfkB domain-containing protein Adenosine kinase activity (GO:0004001), Purine ribonucleoside salvage (GO:0006166)
Down 18,075 C5YXW7 Guanosine nucleotide diphosphate dissociation inhibitor Rab GTPase binding (GO:0017137), small GTPase mediated signal transduction (GO:0007264)
Down 19,332 C5X6V0 similar to Extracellular ribonuclease LE RNA catabolic process (GO:0006401)
Down 19,346 C5YG66 Aminomethyltransferase Aminomethyltransferase activity (GO:0004047), Glycine decarboxylation via glycine cleavage system (GO:0019464)
Down 21,133 C5YG29 similar to 60S ribosomal protein Translation (GO:0006412)
Down 22,396 C5YCD6 Phenylalanine ammonia-lyase L-phenylalanine = NH4+ + trans-cinnamate, Cinnamic acid biosynthetic process (GO:0009800), L-phenylalanine catabolic process (GO:0006559)
Down 22,977 C5WT26 40S ribosomal protein S4 Translation (GO:0006412)
Down 23,733 C5YX57 40S ribosomal protein S4 Translation (GO:0006412)
Down 23,995 C5YU66 similar to Heat shock 70 kDa protein 4 Stress response
Down 24,630 C5YJP1 HATPase_c domain-containing protein Unfolded protein binding (GO:0051082), Response to chlorate (GO:0010157), heat (GO:0009408), salt stress (GO:0009651), water deprivation (GO:0009414)
Down 25,743 C5X255 similar to Formate tetrahydrofolate ligase  
Down 25,986 C5WXD2 similar to Protein TIC110, chloroplastic  
Down 26,465 C5XXT8 Phenylalanine ammonia-lyase L-phenylalanine = NH4+ + trans-cinnamate, Cinnamic acid biosynthetic process (GO:0009800), L-phenylalanine catabolic process (GO:0006559)
Down 28,031 C5XIT6 Pectinesterase [(1 → 4)-α-D-galacturonosyl methyl ester](n) + n H2O = [(1 → 4)-α-D-galacturonosyl](n) + n H+ + n methanol, cell wall modification (GO:0042545)
Down 28,874 C5YMU8 similar to Puromycin-sensitive aminopeptidase  
Down 29,216 C5YPW0 similar to ATP-citrate synthase ATP binding (GO:0005524)
Down 30,618 C5WZ87 similar to Ribosomal protein S9 Translation (GO:0006412)
Down 30,990 C5XI18 S-adenosylmethionine synthase ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine, S-adenosylmethionine biosynthetic process (GO:0006556)
Down 31,330 C5YNT6 S4 RNA-binding domain-containing protein Translation (GO:0006412), Positive regulation of translational fidelity (GO:0045903)
Down 31,631 C5WXA8 NADPH-protochlorophyllide oxidoreductase chlorophyllide a + NADP+ = H+ + NADPH + protochlorophyllide a
Down 31,939 C5WZQ4 similar to 50S ribosomal protein L6 Translation (GO:0006412)
Down 32,283 C5XE18 40S ribosomal protein SA Cytoplasmic translation (GO:0002181), Translation (GO:0006412)
Down 32,318 C5YFQ2 Ribosomal_S17_N domain-containing protein Translation (GO:0006412)
Down 32,520 C5X0S2 Uroporphyrinogen decarboxylase 4 H+ + uroporphyrinogen III = 4 CO2 + coproporphyrinogen III, Protoporphyrinogen IX biosynthetic process (GO:0006782)
Down 32,758 C5YS19 SAM_MPBQ_MSBQ_MT domain-containing protein Methyltransferase activity (GO:0008168)
  1. The OPLS-DA analysis followed by S-plot analysis was carried out to identify proteins from S. bicolor genotypes that showed significant differential abundance. The commonly expressed proteins identified from S. bicolor genotypes in all the treatments namely steady-state and C. partellus induced were considered for this analysis