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Fig. 3 | Proteome Science

Fig. 3

From: iTRAQ-based proteomic analysis of sperm reveals candidate proteins that affect the quality of spermatozoa from boars on plateaus

Fig. 3

Western blot validation. (a) Western blot (WB) analysis of four proteins (CFL1, CYCS, FN1, and GPX4). β-actin was used as a loading control. (b) Statistical results of quantitative comparison of the isobaric tag for relative and absolute quantification (iTRAQ) and WB for the four proteins. The bar chart shows the statistical results of WB. The line chart represents the quantitative comparison of iTRAQ and WB for the four proteins. The data for the WB expression levels are represented as the means ± standard deviation (SD); * Significant difference (P < 0.05), ** extremely significant difference (P < 0.01). TP = Tibetan pig (n = 10); YP = Yorkshire pig (n = 10)

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