Skip to main content
Fig. 1 | Proteome Science

Fig. 1

From: Identification and validation of serum autoantibodies in children with B-cell acute lymphoblastic leukemia by serological proteome analysis

Fig. 1

The overview of the experimental workflow. In brief, we separated total proteins extracted from the three B-ALL cell lines (NALM-6、REH and BALL-1) by 2-DE. Next, one of the three parallel 2-DE gels was visualized by Coomassie blue staining while the others were transferred onto PVDF membrances. Then, the membranes were incubated with mixed serum samples from 20 B-ALL or 20 healthy controls. After a differential analysis between the blots obtained with patients and controls sera, 6 protein spots of interest were excised from the 2-DE gels, digested by trypsin, and identified by mass spectrometry. The autoantibodies of α-enolase and voltage-dependent anion-selective channel protein 1(VDAC1) were further validated by ELISA, respectively. The candidate antigens α-enolase and VDAC1 were further validated in bone marrow by immunohistochemistry, respectively

Back to article page

Proteome Science

ISSN: 1477-5956