Fig. 4
A Regression analysis of the basic fraction 5 ApoE variant (ε4 proxy) with the fractional APOE ε4 gene dosage of the 12 pools: R2 = 0.83, p < 0.0001. Pool symbols are male healthy controls -╳, female healthy controls -▵, male AD -□, female AD -◇. B Regression analysis of total cohort, non-binding α1-antitrypsin (α1AT) variant vs. apoE ε4 proxy: R2 = 0.82, p < 0.0001; Pools are n = 12. Group symbols are: AD - ⊕, HC - +. C Fraction 5 multiplex gels that have not been aligned for spot-matching. Arrows indicate the correlated changes between the ε4 proxy spot (lower end) and the AD significant α1AT variant (upper end). The AD pools are represented by red in the upper three images and by green in the lower images, reflecting the dye reversal, used between these gels. Yellow represents approximately equal contribution of intensity from AD and control proteins. D. Detail from a pair of multiplexed gel images representative of low ApoE ε4 containing pools (panel i) and high ApoE ε4 containing pools (panel ii). The level of α1ACT isoforms correlated with the 34 kDa ApoE ε4 proxy spot that is shown in lower right-hand corners of the panel i and ii images. Regression analysis Pearson correlations: a- p = 0.012, R2 = 0.45; b- p = 0.002, R2 = 0.61; c- p = 0.003, R2 = 0.56; d- p = 0.002, R2 = 0.61; e- p = 0.007, R2 = 0.51; f- p = 0.003, R2 = 0.58. None of the α1ACT spots significantly discriminated the AD group from the HC group. The α1AT spot that significantly discriminated AD from HC pools (3.3 fold, p < 0.02,) is shown in panel ii