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Fig. 7 | Proteome Science

Fig. 7

From: Identification of different proteins binding to Na, K-ATPase α1 in LPS-induced ARDS cell model by proteomic analysis

Fig. 7

STRING protein–protein interaction analysis. A Protein interaction analysis of control-A549 specifically enriched proteins by STRINGdb showed that there were 244 significant enriched interactions among 60 proteins. (p-value: 6.73572309040082e-13). B Protein interaction analysis of LPS-A549 specifically enriched proteins by STRINGdb showed that there were 43 significant enriched interactions among 29 proteins. (p-value: 0.0488221145460772). C Edge color legends. The explanation of protein–protein interaction links. It is divided into two parts: known interactions and predicted interactions. D The binding of Na, K-ATPase α1 to SQSTM1 and CUL4B were verified by endogenous Co-IP. Proteins in whole-cell lysate were used as a positive control (Input). IP: Na, K-ATPase murine monoclonal antibody, abcam, ab2872. Na, K-ATPase α1 group, WB: 1:1000, 100KD. Second antibody: Goat anti-Mouse IgG (Light Chain Specific), HRP Conjugated, S003, 1:5000. SQSTM1 group, WB: SQSTM1 rabbit polyclonal antibody, Proteintech, 18,420–1, 1:10,000, 62KD; Second antibody: Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, S006, 1:5000. CUL4B group, WB: CUL4B rabbit polyclonal antibody, Immunoway, YM5188, 1:1000, 110KD; Second antibody: Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, S006, 1:5000.62KD

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