Fig. 1

Identification of differentially expressed proteins between cells of shRNA-NC and shRNACs-1429. A The identification of the cell model shRNACs-1429 with low expression of Cs. The expression of Cs in HEI-OC1 cells was tested by Western blotting with β-actin as control. The expression level of Cs in shRNACs-1429 cells was remarkably decreased compared with that of shRNA- NC cells. B Schematic diagram of workflow for the iTRAQ-based approach. Total proteins were extracted from shRNA-NC cells and shRNACs-1429 cells, and part of the samples was taken for protein concentration determination and SDS-PAGE detection, and the other part was taken for trypsin enzymolysis and labeling, then the same amount of labeled samples were mixed for chromatographic separation, and finally the sample was analyzed by LC-MS/MS and data analysis. C The expression profiling changes of proteins from HEI-OC1 cells with of shRNA-NC or shRNACs-1429. Vocalno Plot indicated up-regulated and down-regulated proteins. Dots highlighted in red (up-regulated) or green (down-regulated) indicated proteins differentially expressed based on the criteria of ratio > 1.1 (or < 1/1.1) and P-value < 0.05. D The summary of differentially expressed proteins compared with shRNA-NC cells and shRNACs-1429 cells