Fig. 4
From: A combined protein toxin screening based on the transcriptome and proteome of Solenopsis invicta
Sequence alignment, 3D modeling and phylogenetic analysis of Calglandulin. A Putative sequence TRINITY_DN9166_c1_g16 was aligned with the template (PDB ID: 2F2P). At the bottom of columns, asterisks (*) showed conserved positions, colons (:) showed conserved substitutions and points (.) showed non-conserved substitutions. Grey line, green bend, blue banded arrowhead, and red solenoid represent coil, turn, sheet, and helix, respectively. Different fragments were marked by red framework. B 3D modeling was modeled by SWISS-MODEL and viewed by Discovery Studio 4.5. The color grey, green, blue, and red represents coil, turn, sheet, and helix, respectively. The differences of TRINITY_DN9166_c1_g16 compared with the template (PDB ID: 2F2P) were marked with black font. C The phylogenetic tree including sequence TRINITY_ DN9166_c1_g16 and other ten sequences from different species was constructed by using MEGA 7 with the Neighbor-Joining method. Putative sequence, Myrmicinae, and Ponerinae were marked as red, green, and yellow circles, respectively