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  1. In Drosophila melanogaster, the male seminal fluid contains proteins that are important for reproductive success. Many of these proteins are synthesised by the male accessory glands and are secreted into the acce...

    Authors: Michael J Walker, Caroline M Rylett, Jeff N Keen, Neil Audsley, Mohammed Sajid, Alan D Shirras and R Elwyn Isaac

    Citation: Proteome Science 2006 4:9

    Content type: Research

    Published on:

  2. Scientific publications should provide sufficient detail in terms of methodology and presented data to enable the community to reproduce the methodology to generate similar data and arrive at the same conclusi...

    Authors: Martin Latterich

    Citation: Proteome Science 2006 4:8

    Content type: Editorial

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  3. Cerebrospinal fluid (CSF) potentially carries an archive of peptides and small proteins relevant to pathological processes in the central nervous system (CNS) and surrounding brain tissue. Proteomics is especi...

    Authors: Nelson Guerreiro, Baltazar Gomez-Mancilla and Stéphane Charmont

    Citation: Proteome Science 2006 4:7

    Content type: Methodology

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  4. Halobacterium sp. NRC-1 is an extremely halophilic archaeon and has adapted to optimal growth under conditions of extremely high salinity. Its proteome is highly acidic with a median pI of 4.9, a unique character...

    Authors: Hem D Shukla

    Citation: Proteome Science 2006 4:6

    Content type: Research

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  5. In this study we report the effect of AZT on the whole protein expression profile both in the control and the AZT-treated K562 cells, evidenced by two-dimensional gel electrophoresis and peptide mass fingerpri...

    Authors: Gabriele D'Andrea, Anna R Lizzi, Sara Venditti, Laura Di Francesco, Alessandra Giorgi, Giuseppina Mignogna, Arduino Oratore and Argante Bozzi

    Citation: Proteome Science 2006 4:4

    Content type: Research

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  6. Research with clinical specimens is always hampered by the limited availability of relevant samples, necessitating the use of a single sample for multiple assays. TRIzol is a common reagent for RNA extraction,...

    Authors: Tsz-Kwong Man, Yiting Li, Tu Anh Dang, Jianhe Shen, Laszlo Perlaky and Ching C Lau

    Citation: Proteome Science 2006 4:3

    Content type: Methodology

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  7. This study presents the development and application of protein lysate microarray (LMA) technology for verification of presence and quantification of human tissue samples for protein biomarkers. Sub-picogram ra...

    Authors: Anitha Ramaswamy, E Lin, Iou Chen, Rahul Mitra, Joel Morrisett, Kevin Coombes, Zhenlin Ju and Mini Kapoor

    Citation: Proteome Science 2005 3:9

    Content type: Methodology

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  8. The observed molecular weight of a protein on a 1D polyacrylamide gel can provide meaningful insight into its biological function. Differences between a protein's observed molecular weight and that predicted b...

    Authors: Q Rushdy Ahmad, Dat H Nguyen, Mark A Wingerd, George M Church and Martin A Steffen

    Citation: Proteome Science 2005 3:6

    Content type: Methodology

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  9. The analysis of hydrophobic membrane proteins by two-dimensional gel electrophoresis has long been hampered by the concept of inherent difficulty due to solubility issues. We have optimized extraction protocol...

    Authors: Matthew A Churchward, R Hussain Butt, John C Lang, Kimberly K Hsu and Jens R Coorssen

    Citation: Proteome Science 2005 3:5

    Content type: Methodology

    Published on:

  10. A recombinant B. megaterium strain was used for the heterologous production of a glucosyltransferase (dextransucrase). To better understand the physiological and metabolic responses of the host cell to cultivatio...

    Authors: Wei Wang, Rajan Hollmann, Tobias Fürch, Manfred Nimtz, Marco Malten, Dieter Jahn and Wolf-Dieter Deckwer

    Citation: Proteome Science 2005 3:4

    Content type: Research

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  11. Neuroglobin is a hexacoordinated member of the globin family of proteins. It is predominantly localized to various brain regions and retina where it may play a role in protection against ischemia and nitric ox...

    Authors: Begona Casado, Lewis K Pannell, Gail Whalen, Daniel J Clauw and James N Baraniuk

    Citation: Proteome Science 2005 3:2

    Content type: Research

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  12. The structural integrity of recombinant proteins is of critical importance to their application as clinical treatments. Recombinant growth hormone preparations have been examined by several methodologies. In t...

    Authors: Felix Hepner, Edina Cszasar, Elisabeth Roitinger and Gert Lubec

    Citation: Proteome Science 2005 3:1

    Content type: Research

    Published on:

  13. Quantitative proteomics is an emerging field that encompasses multiplexed measurement of many known proteins in groups of experimental samples in order to identify differences between groups. Antibody arrays a...

    Authors: LT Perlee, J Christiansen, R Dondero, B Grimwade, S Lejnine, M Mullenix, W Shao, M Sorette, VT Tchernev, DD Patel and SF Kingsmore

    Citation: Proteome Science 2004 2:9

    Content type: Methodology

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  14. Chaperones (CH) play an important role in tumor biology but no systematic work on expressional patterns has been reported so far. The aim of the study was therefore to present an analytical method for the conc...

    Authors: Jae-Kyung Myung, Leila Afjehi-Sadat, Maureen Felizardo-Cabatic, Irene Slavc and Gert Lubec

    Citation: Proteome Science 2004 2:8

    Content type: Research

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  15. The aim of this study was firstly, to improve and validate a cerebrospinal fluid (CSF) prefractionation method followed by two-dimensional electrophoresis (2-DE) and secondly, using this strategy to investigat...

    Authors: Sara Folkesson Hansson, Maja Puchades, Kaj Blennow, Magnus Sjögren and Pia Davidsson

    Citation: Proteome Science 2004 2:7

    Content type: Research

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  16. Quantitative proteomic analyses have traditionally used two-dimensional gel electrophoresis (2DE) for separation and characterisation of complex protein mixtures. Among the difficulties associated with this ap...

    Authors: Guennadi A Khoudoli, Iain M Porter, J Julian Blow and Jason R Swedlow

    Citation: Proteome Science 2004 2:6

    Content type: Methodology

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  17. Structural genomics (SG) projects aim to determine thousands of protein structures by the development of high-throughput techniques for all steps of the experimental structure determination pipeline. Crucial t...

    Authors: Stéphane Raymond, Nicholas O'Toole and Miroslaw Cygler

    Citation: Proteome Science 2004 2:4

    Content type: Research

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  18. Cu/Zn-superoxide dismutase 1 (SOD1), encoded on chromosome 21, is a key enzyme in the metabolism of reactive oxygen species (ROS) and pathogenetically relevant for several disease states including Down syndrom...

    Authors: Joo-Ho Shin, Jae-Won Yang, Marie Le Pecheur, Jacqueline London, Harald Hoeger and Gert Lubec

    Citation: Proteome Science 2004 2:2

    Content type: Research

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  19. The rapid completion of genome sequences has created an infrastructure of biological information and provided essential information to link genes to gene products, proteins, the building blocks for cellular fu...

    Authors: Joo-Ho Shin, Jae-Won Yang, Jean-François Juranville, Michael Fountoulakis and Gert Lubec

    Citation: Proteome Science 2004 2:1

    Content type: Research

    Published on:

  20. Cerebral microdialysis has been established as a monitoring tool in neurocritically ill patients suffering from severe stroke. The technique allows to sample small molecules in the brain tissue for subsequent ...

    Authors: Martin H Maurer, Christian Berger, Margit Wolf, Carsten D Fütterer, Robert E Feldmann Jr, Stefan Schwab and Wolfgang Kuschinsky

    Citation: Proteome Science 2003 1:7

    Content type: Research

    Published on:

  21. Separation of proteins by two-dimensional gel electrophoresis (2-DE) coupled with identification of proteins through peptide mass fingerprinting (PMF) by matrix-assisted laser desorption ionization time-of-fli...

    Authors: Wei Wang, Jibin Sun, Manfred Nimtz, Wolf-Dieter Deckwer and An-Ping Zeng

    Citation: Proteome Science 2003 1:6

    Content type: Research

    Published on:

  22. Proteomics experiments typically involve protein or peptide separation steps coupled to the identification of many hundreds to thousands of peptides by mass spectrometry. Development of methodology and instrum...

    Authors: Gerard Cagney, Shiva Amiri, Thanuja Premawaradena, Micheal Lindo and Andrew Emili

    Citation: Proteome Science 2003 1:5

    Content type: Research

    Published on:

  23. Hippocampal neural stem cells (HNSC) play an important role in cerebral plasticity in the adult brain and may contribute to tissue repair in neurological disease. To describe their biological potential with re...

    Authors: Martin H Maurer, Robert E Feldmann Jr, Carsten D Fütterer and Wolfgang Kuschinsky

    Citation: Proteome Science 2003 1:4

    Content type: Research

    Published on:

  24. MALDI-TOF-MS has become an important analytical tool in the identification of proteins and evaluation of their role in biological processes. A typical protocol consists of sample purification, separation of pr...

    Authors: Florence Gonnet, Gilles Lemaître, Gilles Waksman and Jeanine Tortajada

    Citation: Proteome Science 2003 1:2

    Content type: Research

    Published on:

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