Figure 4

Significant canonical pathways ( p < 0.05) for proteins differentially expressed in BT474 (black) and SKBR3 (gray) in comparison with 184A1 cell line. The negative of the log₁₀ (p-value) (long dash dot dot) and ratio (number of focus molecules involved in the pathway/total number of molecules in the pathway) are plotted on the primary and secondary Y-axis respectively. Pathways for BT474 are indicated by black columns and the ratio is indicated by a solid line joined by squares. Similarly, pathways for SKBR3 are indicated by gray columns and corresponding ratio is indicated by long dashes joined by circles. (Panel A) Canonical pathways unique to each cell line. (Panel B) Canonical pathways common to both the cell lines. (Panel C) Functional clustering of gene ontology (GO) terms associated with apoptosis for differentially expressed proteins obtained using DAVID. Green region of the heat map indicates corresponding GO association positively reported and the black region indicates the GO association not reported as yet. Gene symbols colored red are down-regulated and colored green are up-regulated in tumor cell lines in comparison with 184A1.