ITRAQ-based quantitative proteomic analysis of processed Euphorbia lathyris L. for reducing the intestinal toxicity

Euphorbia lathyris L., a Traditional Chinese medicine (TCM), is commonly used for the treatment of hydropsy, ascites, constipation, amenorrhea, and scabies. Semen Euphorbiae Pulveratum, which is another type of E...

Authors: Yu Zhang, Yingzi Wang, Shaojing Li, Xiuting Zhang, Wenhua Li, Shengxiu Luo, Zhenyang Sun and Ruijie Nie

In vitro gentamicin exposure alters caveolae protein profile in cochlear spiral ligament pericytes

The aminoglycoside antibiotic gentamicin is an ototoxic drug and has been used experimentally to investigate cochlear damage induced by noise.

Authors: Elisa Ghelfi, Yohann Grondin, Emil J. Millet, Adam Bartos, Magda Bortoni, Clara Oliveira Gomes dos Santos, Humberto J. Trevino-Villarreal, Rosalinda Sepulveda and Rick Rogers

Effects of the complete replacement of fish oil with linseed oil on growth, fatty acid composition, and protein expression in the Chinese mitten crab (Eriocheir sinensis)

The finite marine resources make it difficult for us to obtain enough fish oil (FO) used in aquatic feeds. Another sustainable ingredients should be found to substitute FO. The effects of replacing FO with veg...

Authors: Banghong Wei, Zhigang Yang, Yongxu Cheng, Jianyi Wang and Junyu Zhou

Profiling the interactome of protein kinase C ζ by proteomics and bioinformatics

Protein kinase C ζ (PKCζ), an isoform of the atypical protein kinase C, is a pivotal regulator in cancer. However, the molecular and cellular mechanisms whereby PKCζ regulates tumorigenesis and metastasis are ...

Authors: Chunyu Hou, Yuan Li, Huiqin Liu, Mengjiao Dang, Guoxuan Qin, Ning Zhang and Ruibing Chen

Proteomic identification and characterization of hepatic glyoxalase 1 dysregulation in non-alcoholic fatty liver disease

Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease worldwide. However, its molecular pathogenesis is incompletely characterized and clinical biomarkers remain scarce. The aims of these ...

Authors: Christos Spanos, Elaina M. Maldonado, Ciarán P. Fisher, Petchpailin Leenutaphong, Ernesto Oviedo-Orta, David Windridge, Francisco J. Salguero, Alexandra Bermúdez-Fajardo, Mark E. Weeks, Caroline Evans, Bernard M. Corfe, Naila Rabbani, Paul J. Thornalley, Michael H. Miller, Huan Wang, John F. Dillon…

Effect of ovarian cancer ascites on SKOV-3 cells proteome: new proteins associated with aggressive phenotype in epithelial ovarian cancer

Epithelial ovarian cancer is the second most lethal gynecological cancer worldwide. Ascites can be found in all clinical stages, however in advanced disease stages IIIC and IV it is more frequent and could be ...

Authors: Alfredo Toledo-Leyva, Julio César Villegas-Pineda, Sergio Encarnación-Guevara, Dolores Gallardo-Rincón and Patricia Talamás-Rohana

Proteomic identification of moesin upon exposure to acrolein

Acrolein (allyl Aldehyde) as one of smoke irritant exacerbates chronic airway diseases and increased in sputum of patients with asthma and chronic obstructive lung disease. But underlying mechanism remains unr...

Authors: Pureun-Haneul Lee, Byeong-Gon Kim, Sun-Hye Lee, George D. Leikauf and An-Soo Jang

Chemical proteomics, an integrated research engine for exploring drug-target-phenotype interactions

Authors: Ho Jeong Kwon and Peter Karuso

Comparative proteomic analysis between nitrogen supplemented and starved conditions in Magnaporthe oryzae

Fungi are constantly exposed to nitrogen limiting environments, and thus the efficient regulation of nitrogen metabolism is essential for their survival, growth, development and pathogenicity. To understand ho...

Authors: Yeonyee Oh, Suzanne L. Robertson, Jennifer Parker, David C. Muddiman and Ralph A. Dean

Proteomic analysis of early salt stress responsive proteins in alfalfa roots and shoots

Alfalfa (Medicago sativa) is the most extensively cultivated forage legume in the world, and salinity stress is the most problematic environmental factors limiting alfalfa production. To evaluate alfalfa tissue v...

Authors: Junbo Xiong, Yan Sun, Qingchuan Yang, Hong Tian, Heshan Zhang, Yang Liu and Mingxin Chen

Iron overload down-regulates the expression of the HIV-1 Rev cofactor eIF5A in infected T lymphocytes

Changes in iron metabolism frequently accompany HIV-1 infection. However, while many clinical and in vitro studies report iron overload exacerbates the development of infection, many others have found no corre...

Authors: Carmine Mancone, Alessio Grimaldi, Giulia Refolo, Isabella Abbate, Gabriella Rozera, Dario Benelli, Gian Maria Fimia, Vincenzo Barnaba, Marco Tripodi, Mauro Piacentini and Fabiola Ciccosanti

Examining the influence of specificity ligands and ATP-competitive ligands on the overall effectiveness of bivalent kinase inhibitors

Identifying selective kinase inhibitors remains a major challenge. The design of bivalent inhibitors provides a rational strategy for accessing potent and selective inhibitors. While bivalent kinase inhibitors...

Authors: Margaret L. Wong, Jason Murphy, Edmund Harrington, Carrie M. Gower, Rishi K. Jain, Markus Schirle and Jason R. Thomas

Daptomycin, a last-resort antibiotic, binds ribosomal protein S19 in humans

Daptomycin is a recently introduced, last-resort antibiotic that displays a unique mode of action against Gram-positive bacteria that is not fully understood. Several bacterial targets have been proposed but n...

Authors: Michael P. Gotsbacher, Sungmin Cho, Ho Jeong Kwon and Peter Karuso

Tetrazine ligation for chemical proteomics

Determining small molecule—target protein interaction is essential for the chemical proteomics. One of the most important keys to explore biological system in chemical proteomics field is finding first-class m...

Authors: Kyungtae Kang, Jongmin Park and Eunha Kim

Photo-affinity labeling (PAL) in chemical proteomics: a handy tool to investigate protein-protein interactions (PPIs)

Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific protei...

Authors: Dhiraj P. Murale, Seong Cheol Hong, Md. Mamunul Haque and Jun-Seok Lee

Thermal proteome profiling: unbiased assessment of protein state through heat-induced stability changes

In recent years, phenotypic-based screens have become increasingly popular in drug discovery. A major challenge of this approach is that it does not provide information about the mechanism of action of the hit...

Authors: André Mateus, Tomi A. Määttä and Mikhail M. Savitski

The hepatocyte proteome in organotypic rat liver models and the influence of the local microenvironment

Liver models that closely mimic the in vivo microenvironment are useful for understanding liver functions, capabilities, and intercellular communication processes. Three-dimensional (3D) liver models assembled...

Authors: Lucas T. Vu, Sophia M. Orbach, W. Keith Ray, Margaret E. Cassin, Padmavathy Rajagopalan and Richard F. Helm

Characterisation of the circulating acellular proteome of healthy sheep using LC-MS/MS-based proteomics analysis of serum

Unlike humans, there is currently no publicly available reference mass spectrometry-based circulating acellular proteome data for sheep, limiting the analysis and interpretation of a range of physiological cha...

Authors: Saul Chemonges, Rajesh Gupta, Paul C. Mills, Steven R. Kopp and Pawel Sadowski

The effect of microbial challenge on the intestinal proteome of broiler chickens

In poultry production intestinal health and function is paramount to achieving efficient feed utilisation and growth. Uncovering the localised molecular mechanisms that occur during the early and important per...

Authors: Emily L. O’Reilly, Richard J. Burchmore, Nicholas H. Sparks and P. David Eckersall

Water deficit mechanisms in perennial shrubs Cerasus humilis leaves revealed by physiological and proteomic analyses

Drought (Water deficit, WD) poses a serious threat to extensively economic losses of trees throughout the world. Chinese dwarf cherry (Cerasus humilis) is a good perennial plant for studying the physiological and...

Authors: Zepeng Yin, Jing Ren, Lijuan Zhou, Lina Sun, Jiewan Wang, Yulong Liu and Xingshun Song

Merging clinical chemistry biomarker data with a COPD database - building a clinical infrastructure for proteomic studies

Data from biological samples and medical evaluations plays an essential part in clinical decision making. This data is equally important in clinical studies and it is critical to have an infrastructure that en...

Authors: Jonatan Eriksson, Simone Andersson, Roger Appelqvist, Elisabet Wieslander, Mikael Truedsson, May Bugge, Johan Malm, Magnus Dahlbäck, Bo Andersson, Thomas E. Fehniger and György Marko-Varga

Proteomic profiling of HBV infected liver biopsies with different fibrotic stages

Hepatitis B virus (HBV) is a global health problem, and infected patients if left untreated may develop cirrhosis and eventually hepatocellular carcinoma. This study aims to enlighten pathways associated with ...

Authors: Seyma Katrinli, Kamil Ozdil, Abdurrahman Sahin, Oguzhan Ozturk, Gozde Kir, Ahmet Tarik Baykal, Emel Akgun, Omer Sinan Sarac, Mehmet Sokmen, H. Levent Doğanay and Gizem Dinler Doğanay

Disulfide proteomics of rice cultured cells in response to OsRacl and probenazole-related immune signaling pathway in rice

Reactive oxygen species (ROS) production is an early event in the immune response of plants. ROS production affects the redox-based modification of cysteine residues in redox proteins, which contribute to prot...

Authors: Kazuko Morino, Mayumi Kimizu and Masayuki Fujiwara

Proteomic analysis of microparticles isolated from malaria positive blood samples

Malaria continues to be a great public health concern due to the significant mortality and morbidity associated with the disease especially in developing countries. Microparticles (MPs), also called plasma mem...

Authors: Samuel Antwi-Baffour, Jonathan Kofi Adjei, Francis Agyemang-Yeboah, Max Annani-Akollor, Ransford Kyeremeh, George Awuku Asare and Ben Gyan

Protein profiles of hatchery egg shell membrane

Eggshells which consist largely of calcareous outer shell and shell membranes, constitute a significant part of poultry hatchery waste. The shell membranes (ESM) not only contain proteins that originate from e...

Authors: N. C. Rath, R. Liyanage, S. K. Makkar and J. O. Lay Jr.