Figure 1

Comparison of 2-DE analysis and western blot analysis of different M. tuberculosis bacterial proteins at pH 4-7. 80 μg of total protein lysates from M. tuberculosis were loaded in the first dimension and approximately 1000 spots were detected after silver staining. Proteins identified were indicated by spot number and in Table 1. (A) 2-DE analysis of cell lysates from antibiotic-susceptible strains (a1), multi-drug resistant strains (a2). (B) Western blot analysis of 2-DE maps of M. tuberculosis using pooled serum from patients infected with MDR clinical strains. Protein lysates examined were from sensitive (b1), multi-drug resistant strains (b2). (C) Western blot analysis of 2-DE maps of M. tuberculosis using pooled serum from patients infected with antibiotic-sensitive clinical strains. Protein lysates were from sensitive (c1), multi-drug resistant strains (c2). Gels were electroblotted onto PVDF membranes using a semidry transfer unit. All seras were used at the same dilution to 1:400. Labeled protein spots were excised from corresponding 2DE reference gels run in parallel and subsequently identified using MALDI-TOF-MS and MALDI-TOF-MS/MS analysis.