Figure 3From: Identification of putative biomarkers for the serodiagnosis of drug-resistant Mycobacterium tuberculosis Subcellular localization of M. tuberculosis H37Rv proteins. Lysates from M. tuberculosis H37Rv were fractionated into cytosol (CY), cell membrane (CM), cell wall (CW) and culture filtrate (CF) fractions. These fractions were normalized for total protein content (50 μg) before SDS-PAGE and then electroblotted onto a PVDF membrane and probed with rabbit-antisera raised against Rv0859c, Rv2031c, Rv2145c, Rv3692, or Rv0444c, respectively.Back to article page