Table 1 Immunoreactive protein spots identified by MALDI-TOF-MS
Spot Noa. | NCBI accession no.b | Sanger I.D. | Protein identification | MW/PI | Mascot scorec | GRAVY scored | 2D immunoreactivity | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | Â | Theoretical | Experimental | Â | Â | Sensitive M. tuberculosis | RIF r M. tuberculosis | MDR M. tuberculosis |
M1 | gi|1322434 | Rv1310 | ATP synthase beta chain (atpD) | 53093.32/4.76 | 53061.2/4.86 | 433, 57 | -0.168 | S-serae | All sera | All sera |
M2 | gi|2916918 | Rv0859c | Acyl-CoA thiolase (fadA) | 42413.60/5.14 | 42387.8/5.2 | 181, 32 | 0.035 | R-sera | -- | -- |
M3 | gi|15610828 | Rv3692 | Methanol dehydrogenase regulatory protein(moxR2) | 37903.47/5.51 | 37904.3/5.43 | 192, 20 | -0.002 | -- | R-sera | -- |
M4 | gi|15610165 | Rv3028c | electron transfer flavoprotein (alpha subunit) (fixB, etfA) | 31690.02/4.61 | 31699.8/4.47 | 746, 68 | 0.358 | -- | -- | R-sera |
M5 | gi|2791638 | Rv3040 | Conserved hypothetical protein | 31484.26/4.50 | 31465.7/4.65 | 98, 6 | -0.297 | -- | -- | R-sera |
M6 | gi|2104333 | Rv2145c | antigen 84(wag31) | 28276.23/4.75 | 28260.1/4.80 | 133, 49 | -0.726 | -- | -- | R-sera |
M7 | gi|15607779 | Rv0639 | Transcription antitermination protein(nusG) | 25413.54/4.59 | 25431.0/4.70 | 175, 23 | -0.180 | -- | -- | R-sera |
M8 | gi|15607585 | Rv0444c | Conserved hypothetical protein | 23882.15/5.82 | 23883.0/5.81 | 75, 13 | 0.062 | -- | R-sera | -- |
S1 | gi|2896768 | Rv2031c | Heat Shock Protein hspX (14-kDa antigen) | 16226.30/4.90 | 16217.2/5.0 | 209, 35 | -0.52 | -- | S-sera | -- |