Table 3 Primers sets for genes amplified by PCR
Gene | Primer sequences(5'-3') | Restriction site used |
|---|---|---|
Rv1310 | 5' CGAATTC ATGACTACCACTGCCGAAAAG 3' | EcorI |
| Â | 5' TATAAGCTT TCACAGCTTGGCGCCGAGAC 3' | HinD III |
Rv3028c | 5' TAGGATCC ATGGCTGAAGTACTGGTGCTC 3' | BamHI |
| Â | 5' TATAAGCTT CTAGCCCTTGCGGGCCTTG 3' | HinD III |
Rv3040 | 5' TAGGATCC ATGAATTCACCTCGCGAGCC 3' | BamHI |
| Â | 5' TATAAGCTT TCACAGCGGCCACCCGGTC 3' | HinD III |
Rv2145c | 5' TAGGATCC ATGCCGCTTACACCTGCCG 3' | BamHI |
| Â | 5' TAGAGCTC CTAGTTTTTGCCCCGGTTGAAT 3' | SacI |
Rv0639 | 5' TAGGATCC GTGACTACCTTCGACGGTG 3' | BamHI |
| Â | 5' GACAAGCTT CTAGATCTTGGAGACTTGGCC 3' | HinD III |
Rv3692 | 5' TAGGATCC GTGACACAGTCCGCGTCCAAC 3' | BamHI |
| Â | 5' TGTAAGCTT CTAGCGGGGCACCGGAACC 3' | HinD III |
Rv0444c | 5' TGGATCC ATGACTGAACATACCGATTTTGAG 3' | BamHI |
| Â | 5' TATAAGCTT TCACCCGAGCGGCAGCTCGGC 3' | HinD III |
Rv2031c | 5' TAGGATCC ATGGCCACCACCCTTCCCGT 3' | BamHI |
| Â | 5' GCGAAGCTT TCAGTTGGTGGACCGGATCTG 3' | HinD III |
Rv0859c | 5' ATGGATCC ATGTCCGAAGAAGCCTTCATCTAC 3' | BamHI |
| Â | 5' TATAAGCTT TTAAACCCTCTCGATGATCGTCGC 3' | HinD III |