Figure 2
From: Characterisation of the vitreous proteome in proliferative diabetic retinopathy
Identification of proteins by MS and MS/MS. As an example, the identification of phosphatidylethanolamine-binding protein 1 is represented here. The protein from spot 1010 was excised from the gels and digested with trypsin, and the resulting peptides were analysed using a MALDI-TOF/TOF mass spectrometer. (A) The MS spectrum and (B) the MS/MS spectrum for the peptide CDEPILSNR. (C) The MS/MS spectrum for the peptide LYTLVLTDPDAPSR. (D) The MS/MS spectrum for the peptide GNDISSGTVLSDYVGSGPPK. The chart represents m/z (horizontal) versus intensity (vertical). The spectra for the tryptic peptides of PEBP 1 were annotated using the GPS explorer software, v3.6 and the Mascot search engine, v2.1 with the UniprotKB/SwissProt database.