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Table 4 Differential abundance changed proteins in kidney from chicken infected with IBV ck/CH/LDL/97I P5 and ck/CH/LDL/97I P115

From: Proteomics analysis of differentially expressed proteins in chicken trachea and kidney after infection with the highly virulent and attenuated coronavirus infectious bronchitis virus in vivo

Master IDa

Protein description

Accession no.b

Protein score

Protein score CI%c

Mw (Da)

pI

Average ratio and p-value

       

P 5 -infected/control

P 115 -infected/control

       

4 dpi

7 dpi

14 dpi

21 dpi

4 dpi

7 dpi

14 dpi

21 dpi

       

ratio

p

ratio

p

ratio

p

ratio

p

ratio

p

ratio

p

ratio

p

ratio

p

496

aldehyde dehydrogenase 4 family, member A1

gi|118101121

1010

100

112936

5.4

  

0.56

0.009

      

0.85

0.031

    

662

aldehyde dehydrogenase 4 family, member A1

gi|118101121

890

100

112936

5.4

    

1.03

0.84

      

1.59

0.015

  

452

alpha-enolase

gi|46048768

353

100

47617.5

6.17

1.25

0.14

      

1.7

0.003

      

821,

1088

alpha-tropomyosin of smooth muscle (TPM1)

gi|833618

94

99.999

32962.7

4.67

  

2.22

0.003

      

1.16

0.46

    

1977

apolipoprotein A-I (APOA1)

gi|211159

703

100

30673.2

5.58

      

0.97

0.31

      

1.58

0

1262

chain A, crystal structures of chicken annexin V in complex with Ca2+ (ANXA5)

gi|62738641

728

100

36158.6

5.61

  

2.04

0.004

      

1.1

0.75

    

983,

784

chain C, crystal structure of native chicken fibrinogen

gi|8569623

921

100

47485.9

5.4

    

1.69

0.002

1.24

0.034

    

1.07

0.35

0.76

0.006

1926

low molecular weight phosphotyrosine proteinphosphatase (TCP1)

gi|86129490

304

100

18640.2

6.81

  

6.34

0.045

      

0.74

0.16

    

1847

manganese-containing superoxide dismutase precursor (MNSOD)

gi|12034955

258

100

25158.7

8.6

1.52

0.015

      

1.12

0.022

      

1970

nucleoside diphosphate kinase

gi|2827446

445

100

17542

7.11

  

4.68

0.003

      

1.48

0.81

    

293

phosphoenolpyruvate carboxykinase (EC 4.1.1.32)

gi|212538

492

100

70224.3

6.1

1.15

0.047

      

1.78

0

      

665

similar to aflatoxin aldehyde reductase

gi|118101125

286

100

36943.4

6.76

  

2.61

0.004

      

1.28

0.059

    

789

similar to betaine homocysteine methyltransferase

gi|50755288

1010

100

45552.1

7.56

  

2.51

       

1.41

     

582

glutamate dehydrogenase 1, mitochondrial

gi|118534

339

100

56075.4

8.48

  

1.74

0.051

      

1.06

0.17

    

1461,

2026

similar to methyltransferase 24 (MET24)

gi|50750103

556

100

30640.5

6

0.72

0.002

  

1.25

0.074

  

1.09

0.066

  

0.42

0

  

987

sulfotransferase

gi|45384226

265

100

36333.5

5.89

  

4.53

0.01

      

0.98

0.85

    

1650

triosephosphate isomerase (EC 5.3.1.1)

gi|212774

1290

100

26831.9

6.71

1.51

0.009

      

1.07

0.43

      
  1. a) Match ID represents unique numbers assigned to each spot in the matched standard of Ettanâ„¢ DeCyder 2D v6.5 software
  2. b) Accession no. is the MASCOT result of MALDI-TOF/TOF searched from the NCBInr database
  3. c) The criterion for successful identification of a protein is a protein score confidence interval (C.I. %) for PMF and MS/MS data ≥ 95%