Comparative proteomic analysis of early salt stress-responsive proteins in roots of SnRK2 transgenic rice

Nam, Myung Hee; Huh, Sun Mi; Kim, Kyung Mi; Park, Woong Woong; Seo, Jong Bok; Cho, Kun; Kim, Dool Yi; Kim, Beom Gi; Yoon, In In

doi:10.1186/1477-5956-10-25

Proteome Science

Table 2 The potential SnRK2 phosphorylation sites found in protein spots showing more than 1.5 fold change in OSRK1 transgenic rice roots at unstressed condition

From: Comparative proteomic analysis of early salt stress-responsive proteins in roots of SnRK2 transgenic rice

Spot^a	Protein Identification	NCBI Accession #	Potential recognition motifs (SnRK2)^b	S^c	T/W^d
5	Elongation factor Tu	gi\|21685576	RRILS, RGIT	U	I
27	Aspartate-semialdehyde dehydrogenase	gi\|113549818	RRPSS, FLRVIS	U	U
61	Fructose-bisphosphate aldolase	gi\|790970	RFAS	D	D
67	Aspartate aminotransferase	gi\|29468084	RVAT, RVKS	U	U
68	Putative isovaleryl-CoA dehydrogenase	gi\|113578072	RRLYS, LVRHGS	U	U
69	Putative acetyl-CoA C-acetyltransferase	gi\|113630918	RSSS, RKGS	U	U
76	Dihydrolipoamide dehydrogenase precursor	gi\|113532449	RLGS, RFMT	U	I
77	Transketolase	gi\|227468492	RNLS, RVVS	U	U
79	Simillar to enolase	gi\|115478881	RAAT, RNQS, RMGS	U	U
82, 227	Putative inorganic pyrophosphatase	gi\|113537770	RRRCSLRTNS, RKVS	D	D
112	Glutathione S-transferase	gi\|31433227	Not found	U	D
112	Proteasome subunit beta type 2	gi\|17380213	RRAYT	U	D
112	Putative chaperonin21 precursor	gi\|51090748	RVCS, RRPS	U	D
116	Triose phosphate isomerise	gi\|553107	Not found	U	U
124	Formate dehydrogenase, mitochondrial precursor	gi\|21263611	Not found	U	U
125	Glutathione S-transferase II	gi\|3746581	MARPSS	U	U
134	Oryzacystatin	gi\|1280613	Not found	D	D
134, 214, 227	15 kda organ-specific salt-induced protein	gi\|256638	FGRSGT	D	D
160	Ascorbate peroxidise	gi\|1321661	Not found	D	D
200	Peroxidase	gi\|257657027	IDRAKS, RNDS	U	U
200	Glutamine synthetase	gi\|124052115	RTLS, RRLT, RHET, RGAS, RPAS	U	U
202, 265	Putative glyoxalase II	gi\|113533338	RARPIS	U	U
206	Glyoxalase I	gi\|113623141	IQRGPT	U	U
213	Thioredoxin type H	gi\|82407383	Not found	U	U
225	Calreticulin	gi\|6682833	RARSSS	D	D
258	Putative enoyl-ACP reductase	gi\|113623526	IGRALS, RAMS, RVNT	U	U
259	Methylmalonate semi-aldehyde dehydrogenase	gi\|113610618	LLRSGS, RVQS, RDAT, VKRASS	D	D
275,277	2,3-bisphosphoglycerate-independent phosphoglycerate mutase	gi\|257353836	RSET, VKRNKS	U	U
276, 278	DnaK-type molecular chaperone precursor	gi\|257307253	FARTFS, RQAT, INRNTT	U	U
292, 293	Phosphoglyceromutase	gi\|257353838	RIAS, RAET, VKRNKS	U	U
314	Glutamate dehydrogenase	gi\|33242905	LTRVFT	U	U
314	Aspartate aminotransferase	gi\|215768565	RLPT	U	U
315, 318	Glyceralde-3-phosphate dehydrogenase	gi\|968996	RAAS, RVPT	U	U
385, 67	Phosphoglycerate_kinase	gi\|113596357	Not found	U	U
383	Translation initiation factor 5A	gi\|113611710	RLPT	U	I
386	Pathogen-related protein	gi\|16589076	Not found	U	U
390	Aldehyde dehydrogenase	gi\|8163730	RRGSS, LQRFST, RVGT	U	U
392	Hypothetical protein	gi\|14192878	VLRLRS, RYAT	U	U

^aSpot numbers correspond to the spots in Figure 2
^bPotential phosphorylation motifs for SnRK2 kinase, RXX(S/T) were analyzed. Motifs with favouring amino acids (L, I, V, M, F, R) at -5 position for CDPK/SnRK2 were marked as bold characters according to Vlad et al. (2008). Among these, the highly preferred motifs, (L/I)XRXX(S/T) were underlined
^cUp (U) or down (D)-regulated protein spots in wild type rice roots after NaCl treatment for 3 h or 7 h
^dUp (U) or down (D)-regulated or induced (I) protein spots in OSRK1 transgenic rice roots compared to wild type roots at 3 h or 7 h control

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ISSN: 1477-5956

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