Figure 6

The amino acid sequence of Lotgi1|159331, an acidic Gln-rich protein with multiple sequence repeats. The predicted secretion signal sequence (aa1–19) is underlined. Sequences covered by MS/MS are in red, the peptide giving rise to the spectrum below is in bold italics and underlined. The theoretical pI for this sequence is 4.0, and the amino acid composition includes 27% Gln, 13% leu and 12% Thr. Eight 21aa-long Gln-rich sequence repeats are alternately shaded grey and yellow. No peptides from the repeat region were obtained because of the lack of tryptic cleavage sites. The doubly charged peptide with m/z 642.80 and a mass deviation of 0.6 ppm had a PEP of 6.2E-09.